Recombinant Chicken Anti-Fusarium Antibody (FvSG7) (CAT#: FAMAB-0191-CN)

A phage-displayed chicken antibody, FvSG7, binds on the surface antigen of conidiospores and the mycelia of F. verticillioides. This antibody binds to Fusarium with high affinity.


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IF

Figure 1 Photomicrographs of F. verticillioides immunostained with the Fusarium-specific FvSG7.

Figure 1 Photomicrographs of F. verticillioides immunostained with the Fusarium-specific FvSG7.

(a and c) Conidiospores of F. verticillioides were examined under a bright-field microscope. (e and g) Hyphae of F. verticillioides were examined under a bright-field microscope. (b and f) The same slides shown in panels (a and e) were examined under Cy3 with FvSG7 antibody.

WB

Figure 2 Antigen-binding properties of scFv.

Figure 2 Antigen-binding properties of scFv.

Immunoblot of SCWPs from F. verticillioides detected with FvSG7 antibody.

ELISA

Figure 3 The sensitivity and detection limits of scFv antibody and scFv–AP fusion determined with different concentrations of F. verticillioides.

Figure 3 The sensitivity and detection limits of scFv antibody and scFv–AP fusion determined with different concentrations of F. verticillioides.

100 μL of the fungus was ground and diluted in PBS atindicated concentrations and added into plate wells for ELISA detection with 200 nM purified scFv antibody.

SPR

Figure 4 Binding kinetics (KD) to SCWPs of F. verticillioides of FvSG7 determined.

Figure 4 Binding kinetics (KD) to SCWPs of F. verticillioides of FvSG7 determined.

Association and dissociation constants of FvSG7 antibody were determined separately by SPR measurements using BIAcore 3000.


Specifications

  • Immunogen
  • A representative isolate of F. verticillioides wh1-2
  • Host Species
  • Chicken
  • Derivation
  • Phage display library
  • Type
  • Chicken IgG
  • Specificity
  • F. verticillioides Fusarium
  • Species Reactivity
  • F. verticillioides
  • Clone
  • FvSG7
  • Applications
  • Used for immunoassay techniques such as: Enzyme Linked Immunoabsorbent Assay; Immunofluorescence Microscopy
  • Related Disease
  • Fusarium ear rot and Fusarium head blight

Product Property

  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Target

  • Alternative Names
  • Fusarium

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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