A phage-displayed chicken antibody, FvSG7, binds on the surface antigen of conidiospores and the mycelia of F. verticillioides. This antibody binds to Fusarium with high affinity.
Figure 1 Photomicrographs of F. verticillioides immunostained with the Fusarium-specific FvSG7.
(a and c) Conidiospores of F. verticillioides were examined under a bright-field microscope. (e and g) Hyphae of F. verticillioides were examined under a bright-field microscope. (b and f) The same slides shown in panels (a and e) were examined under Cy3 with FvSG7 antibody.
Figure 2 Antigen-binding properties of scFv.
Immunoblot of SCWPs from F. verticillioides detected with FvSG7 antibody.
Figure 3 The sensitivity and detection limits of scFv antibody and scFv–AP fusion determined with different concentrations of F. verticillioides.
100 μL of the fungus was ground and diluted in PBS atindicated concentrations and added into plate wells for ELISA detection with 200 nM purified scFv antibody.
Figure 4 Binding kinetics (KD) to SCWPs of F. verticillioides of FvSG7 determined.
Association and dissociation constants of FvSG7 antibody were determined separately by SPR measurements using BIAcore 3000.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-376 | Recombinant Anti-Fusarium Antibody | ELISA, WB, IF, FuncS | IgG |
FAMAB-0116YC | Recombinant Chicken Anti-Fusarium Antibody (CWP2) | ELISA, IF | Chicken IgG |
VS-0422-LC4 | Mouse Anti-Fusarium Recombinant Antibody (VS-0422-LC4) | ELISA, IB | Mouse IgG |
VS-0422-LC5 | Chicken Anti-Fusarium Recombinant Antibody (clone GS3) | ELISA | Chicken IgY |
VS-0422-LC6 | Chicken Anti-Fusarium Recombinant Antibody (clone MSP5) | ELISA | Chicken IgY |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-376-F(E) | Recombinant Anti-Fusarium Antibody Fab Fragment | WB, FACS, FuncS | Fab |
MHH-376-F(E) | Recombinant Human Anti-Fusarium Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
FAMAB-0116-YC-F(E) | Recombinant Chicken Anti-Fusarium Antibody Fab Fragment (CWP2) | ELISA, IF | Chicken Fab |
FAMAB-0191-CN-F(E) | Recombinant Chicken Anti-Fusarium Antibody Fab Fragment (FvSG7) | ELISA, IF | Chicken Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-376-S(P) | Recombinant Anti-Fusarium Antibody scFv Fragment | IF, Neut, FuncS | scFv |
MHH-376-S(P) | Recombinant Human Anti-Fusarium Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
FAMAB-0116-YC-S(P) | Recombinant Chicken Anti-Fusarium Antibody scFv Fragment (CWP2) | ELISA, IF | Chicken scFv |
FAMAB-0191-CN-S(P) | Recombinant Chicken Anti-Fusarium Antibody scFv Fragment (FvSG7) | ELISA, IF | Chicken scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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