Rabbit Anti-GATA3 Recombinant Antibody (clone 2A9)
CAT#: ZG-0011U
The purified Rabbit Anti-GATA3 Recombinant Antibody (clone 2A9) displayed high specificity against the GATA3 protein of human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-GATA3 Antibody (ZG-0011U) in WB
Positive WB detected in: RAW264.7 whole cell lysate. All lanes: GATA3 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 49 kDa
Observed band size: 48 kDa
IHC
Figure 2 Rabbit Anti-GATA3 Antibody (ZG-0011U) in IHC
IHC image of ZG-0011U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29730/295_B8_1_selected.jpg
Normal Tissue
Figure 2 High expression in placenta.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_1_7_val_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_7_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_B_3_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, MCF7, RT4, SH-SY5Y, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107485-GATA3
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Specifications
- Immunogen
- A synthesized peptide derived from human GATA3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GATA3
- Species Reactivity
- Human
- Clone
- 2A9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hypoparathyroidism, Sensorineural Deafness, And Renal Disease and End Stage Renal Disease
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user. WB:1:500-1:5000, IHC:1:50-1:200
Target
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GATA3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2304z | Mouse Anti-GATA3 Recombinant Antibody (clone 13H6) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0377J | Mouse Anti-GATA3 Recombinant Antibody (ZG-0377J) | WB, ELISA | Mouse IgG |
| ZG-0378J | Mouse Anti-GATA3 Recombinant Antibody (ZG-0378J) | WB, IHC, ELISA | Mouse IgG |
| VS3-CJ803 | Mouse Anti-GATA3 Recombinant Antibody (VS3-CJ803) | WB, IF, FC | Mouse IgG2b, κ |
| VS3-CJ804 | Rabbit Anti-GATA3 (phospho S308) Recombinant Antibody (VS3-CJ804) | WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2742 | Anti-GATA3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2743 | Anti-Mouse GATA3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2744 | Anti-Rat GATA3 Immunohistochemistry Kit | IHC |
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