Rabbit Anti-GC Recombinant Antibody (clone R03-5G8)
CAT#: VS3-FY1935
Clone R03-5G8 is a rabbit recombinant monoclonal antibody that detects human GC. It cross-reacts with mouse, rat. The antibody is recommended for detection of GC protein by WB, ICC/IF. The vitamin D binding protein antibody has been teted positive by western blot analysis of vimentin (Phospho-Ser72) in rat brain lysates and immunocytochemistry analysis of vitamin D binding protein (green) in HEPG2.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19855/47306_A_9_3.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19855/47306_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1526/4388_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HL-60, RH-30, SiHa, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145321-GC
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WB
Figure 1 Anti-GC Antibody (VS3-FY1935) in WB.
The Vimentin (Phospho-Ser72) expression in rat Brain lysates were detected using the anti-Phospho-Vimentin (Ser72) by western blot assay.
ICC
Figure 2 Anti-GC Antibody (VS3-FY1935) in ICC.
Immunocytochemistry staining of Vitamin D Binding Protein (green) in HEPG2 using the anti-Vitamin D Binding Protein antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human vitamin D binding protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GC
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5G8
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Rickets and Blastomycosis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- GC, Vitamin D Binding Protein; Vitamin D-Binding Protein-Macrophage Activating Factor; Group-Specific Component (Vitamin D Binding Protein); Gc Protein-Derived Macrophage Activating Factor; Gc-Globulin; DBP-Maf; Gc-MAF; GcMAF; DBP; VDB; Epididymis Secretory Protein Li 51
- Gene ID
- 2638
- UniProt ID
- P02774
- Sequence Similarities
- Belongs to the ALB/AFP/VDB family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Allele GC*1S is O-glycosylated at Thr-436 (PubMed:20079467).
The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine.
This conversion is thought to produce a macrophage-activating factor (Gc-MAF).
Only a minor proportion of plasma GC is O-glycosylated (PubMed:17360250).
The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.
(P02774-VTDB_HUMAN)
Glycosylation at Asn288 (NX_P02774 [NX_P02774-1])
Modification sites at PhosphoSitePlus (P02774)
Glycosylation from GlyConnect VTDB_HUMAN (619)
Glycosylation from GlyGen (P02774) 3 sites, 2 N-linked glycans (1 site), 2 O-linked glycans (1 site)
- Protein Refseq
- XP_006714240.1; XP_054205648.1; NP_000574.2; NP_001191235.1; NP_001191236.1
- Function
- Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOB-1693z | Mouse Anti-GC Recombinant Antibody (clone 7B11) | WB, IHC | Mouse IgG1 |
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| VS3-WK534 | Rabbit Anti-GC Recombinant Antibody (clone JM10-36) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0955MZ | Chicken Anti-GC Polyclonal IgY | ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1412 | Hi-Affi™ Rabbit Anti-GC Recombinant Antibody (clone DS1412AB), Biotin | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY906 | Anti-GC Immunohistochemistry Kit | IHC | |
| VS-0525-XY2759 | Anti-Human GC Immunohistochemistry Kit | IHC |
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