Rabbit Anti-GC Recombinant Antibody (clone R03-5G8) (CAT#: VS3-FY1935)

• Immunogen
• Synthetic peptide of human vitamin D binding protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat GC

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R03-5G8

• Applications
• Western Blot, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.

• Related Disease
• Rickets and Blastomycosis

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200

• Alternative Names
• GC, Vitamin D Binding Protein; Vitamin D-Binding Protein-Macrophage Activating Factor; Group-Specific Component (Vitamin D Binding Protein); Gc Protein-Derived Macrophage Activating Factor; Gc-Globulin; DBP-Maf; Gc-MAF; GcMAF; DBP; VDB; Epididymis Secretory Protein Li 51

• Gene ID
• 2638

• UniProt ID
• P02774

• Sequence Similarities
• Belongs to the ALB/AFP/VDB family.

• Cellular Localization
• Secreted

• Post Translation Modifications
• Allele GC*1S is O-glycosylated at Thr-436 (PubMed:20079467).
  The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine.
  This conversion is thought to produce a macrophage-activating factor (Gc-MAF).
  Only a minor proportion of plasma GC is O-glycosylated (PubMed:17360250).
  The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.
  (P02774-VTDB_HUMAN)
  Glycosylation at Asn288 (NX_P02774 [NX_P02774-1])
  Modification sites at PhosphoSitePlus (P02774)
  Glycosylation from GlyConnect VTDB_HUMAN (619)
  Glycosylation from GlyGen (P02774) 3 sites, 2 N-linked glycans (1 site), 2 O-linked glycans (1 site)

• Protein Refseq
• XP_006714240.1; XP_054205648.1; NP_000574.2; NP_001191235.1; NP_001191236.1

• Function
• Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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