Rabbit Anti-GFP Recombinant Antibody (VS13-YC445)

CAT#: VS13-YC445

The Rabbit Anti-GFP Recombinant Antibody is specific for GFP. GFP is an energy-transfer acceptor which transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. The anti-GFP antibody can be used for Western blot (1/5000-1/10000), Immunohistochemistry-paraffin (1/200-1/500), Immunocytochemistry (1/100-1/500), Immunofluorescence (1/100-1/500), Immunoprecipitation (2-5 µg per 500 μL of cell lysate).

Specifications

  • Immunogen
  • Recombinant A. victoria GFP, aa 1-50.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • GFP
  • Species Reactivity
  • Aequorea victoria
  • Applications
  • WB, ICC, IF, IHC-P, IP
  • Conjugate
  • NONE

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
  • Preservative
  • 0.05% Sodium azide
  • Storage
  • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Shipping with ice pack.

Applications

  • Application Notes
  • Western blot (WB): 1/5000-1/10000
    Immunohistochemistry-paraffin (IHC-P): 1/200-1/500
    Immunocytochemistry (ICC): 1/100-1/500
    Immunofluorescence (IF): 1/100-1/500
    Immunoprecipitation (IP): 2-5 µg per 500 μL of cell lysate

Target

  • Alternative Names
  • Green fluorescent protein
  • Long Name
  • Green fluorescent protein
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

See other products for "GFP"

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PFBZ-050 Mouse Anti-GFP Recombinant Antibody (clone 11G10); Fab Fragment ELISA, WB Mouse Fab
CAT Product Name Application Type
PSBZ-050 Mouse Anti-GFP Recombinant Antibody (clone 11G10); scFv Fragment ELISA, WB Mouse scFv
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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