AibGenesis™ Rabbit Anti-GLS Recombinant Antibody (AibG-Rb-GL1483)
CAT#: VS-0326-XY308
This recombinant rabbit antibody provides robust detection of GLS, a mitochondrial enzyme converting glutamine to glutamate. It is reactive across species and supports studies in metabolism and neurotransmission. Dysregulation may impact cellular energy.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/1509_E1_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/75513_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/75513_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/75513_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/75513_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Pachytene spermatocytes Staining: Low Intensity: Weak Quantity:>75% Preleptotene spermatocytes Staining: Low Intensity: Weak Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36223/75513_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115419-GLS
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- GLS
- Species Reactivity
- Human
- Clone
- AibG-Rb-GL1483
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Purified
- Format
- Liquid
- Buffer
- PBS, pH 7.4, 150mM NaCl and 50% glycerol.
- Preservative
- 0.02% New type preservative N
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:100-1:200, Flow Cytometry: 1:50-1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GLS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1746z | Mouse Anti-GLS Recombinant Antibody (clone 29A8) | WB, ELISA, IF, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0765CT | Recombinant Mouse anti-Human GLS Monoclonal antibody (5C4) | ELISA, FC, ICC, IF, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1456 | Hi-Affi™ Rabbit Anti-GLS Recombinant Antibody (clone DS1456AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0675-CN | Rabbit Anti-GLS Recombinant Antibody (clone CBACN-246) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ186 | Rabbit Anti-GLS Recombinant Antibody (VS3-CJ186) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY595 | Recombinant Rabbit Anti-GLS Antibody (clone R08-3D8) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2832 | Anti-GLS Immunohistochemistry Kit | IHC |
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