Rabbit Anti-GOLM1 Recombinant Antibody (clone R09-9E1) (CAT#: VS3-XY3370)
The rabbit recombinant antibody specifically binds to human GOLM1 (Golgi membrane protein 1). It also has cross-reactivity against mouse and rat GOLM1. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10638/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10638/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11929/94_G3_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11929/94_G3_2_selected.jpg
Figure 3 Stomach 1
(High expression in stomach 1)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10638/28255_A_5_1_val_selected.jpg
(High expression in stomach 1)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10638/28255_A_5_1_val_selected.jpg
Figure 4 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_4_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_4_1.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_7_4.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_A_5_6.jpg
Figure 8 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15341/37233_B_7_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (U-266/70)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135052-GOLM1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135052-GOLM1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GOLM1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-9E1
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Autoimmune Hepatitis; Liver Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
- Gene ID
- 51280
- UniProt ID
- Q8NBJ4
- Long Name
- Golgi membrane protein 1
- Cellular Localization
- Golgi apparatus, cis-Golgi network membrane
- Post Translation Modifications
- Glycosylated. (Q8NBJ4-GOLM1_HUMAN)
Phosphorylation sites are present in the extracellular medium. (Q8NBJ4-GOLM1_HUMAN)
Glycosylation at Asn109, Thr111, Asn144, Thr218, Ser231, Ser233, Thr235, Ser239, and Asn398 (NX_Q8NBJ4 [NX_Q8NBJ4-1])
Ubiquitination at Lys125 (NX_Q8NBJ4 [NX_Q8NBJ4-1])
Modification sites at PhosphoSitePlus (Q8NBJ4)
Glycosylation from GlyConnect GOLM1_HUMAN (1281)
Glycosylation from GlyGen (Q8NBJ4) 18 sites, 32 N-linked glycans (2 sites), 4 O-linked glycans (14 sites)
- Protein Refseq
- NP_057632.2; NP_808800.1
- Function
- Cellular response protein to viral infection.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GOLM1"
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-189CT-F(E) | Mouse Anti-GOLM1 Recombinant Antibody; Fab Fragment (TAB-189CT-F(E)) | ELISA, WB, EIA | Mouse Fab |
| TAB-190CT-F(E) | Mouse Anti-GOLM1 Recombinant Antibody; Fab Fragment (TAB-190CT-F(E)) | ELISA, WB, EIA | Mouse Fab |
| TAB-191CT-F(E) | Mouse Anti-GOLM1 Recombinant Antibody; Fab Fragment (TAB-191CT-F(E)) | ELISA, WB, EIA | Mouse Fab |
| TAB-192CT-F(E) | Mouse Anti-GOLM1 Recombinant Antibody; Fab Fragment (TAB-192CT-F(E)) | ELISA, WB, EIA | Mouse Fab |
| TAB-193CT-F(E) | Mouse Anti-GOLM1 Recombinant Antibody; Fab Fragment (TAB-193CT-F(E)) | ELISA, WB, EIA | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1475 | Hi-Affi™ Rabbit Anti-GOLM1 Recombinant Antibody (clone DS1475AB) | FC, WB, IHC-P | Rabbit IgG |
| MOR-1476 | Hi-Affi™ Rabbit Anti-GOLM1 Recombinant Antibody (clone DS1476AB), FITC | FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-095CQ | Mouse Anti-GOLM1 Recombinant Antibody (clone YC076) | IP, WB, IHC-P, ELISA, IF, IP | Mouse IgG1, κ |
| MRO-0684-CN | Rabbit Anti-GOLM1 Recombinant Antibody (clone JA63-03) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0689J | Rabbit Anti-GOLM1 Recombinant Antibody (clone 9C2) | ELISA, WB, FC | Rabbit IgG |
| VS3-QX471 | Mouse Anti-GOLM1 Recombinant Antibody (clone 3C1) | IHC | |
| VS3-XY774 | Mouse Anti-GOLM1 Recombinant Antibody (clone 2E4) | IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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