CytoStream™ Rabbit Anti-GPER1 Polyclonal Antibody (VS13-YC460)
CAT#: VS13-YC460
The CytoStream™ Rabbit Anti-GPER1 Polyclonal Antibody is specific for human, mouse and rat GPER1. GPER1 encodes a membrane multi-pass protein in ER, associated with estrogen-induced signaling, impacting biological processes like bone development and fertility through non-genomic pathways. The anti-GPER1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 µg per test).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16718/1954_C1_62_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_5_6.jpg
Normal Tissue
Figure 8 Placenta
Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_2_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AN3-CA, Daudi, fHDF/TERT166, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164850-GPER1
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Specifications
- Immunogen
- Recombinant human GPR30 (aa 251-375) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GPER1
- Species Reactivity
- Human, Mouse, Rat, Chicken (Predicted), Cow (Predicted), Horse (Predicted), Rabbit (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 µg per test
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- G Protein-Coupled Estrogen Receptor 1; Flow-Induced Endothelial G-Protein Coupled Receptor 1; Lymphocyte-Derived G-Protein Coupled Receptor; Chemoattractant Receptor-Like 2; G Protein-Coupled Receptor 30; Membrane Estrogen Receptor; IL8-Related Receptor DRY12; GPCR-Br; CMKRL2; LyGPR; GPR30; DRY12;
- Gene ID
- 2852
- UniProt ID
- Q99527
- Long Name
- G Protein-Coupled Estrogen Receptor 1
- Cellular Localization
- Cell membrane; Endoplasmic reticulum membrane; Golgi apparatus membrane
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GPER1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-415 | Recombinant Anti-human GPER1 Antibody | WB, IP, FuncS | IgG |
| MHH-415 | Recombinant Human Anti-human GPER1 Antibody | WB, Neut, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-415-F(E) | Recombinant Anti-human GPER1 Antibody Fab Fragment | WB, RIA, FuncS | Fab |
| MHH-415-F(E) | Recombinant Human Anti-human GPER1 Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-415-S(P) | Recombinant Anti-human GPER1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| MHH-415-S(P) | Recombinant Human Anti-human GPER1 Antibody scFv Fragment | FC, Neut, Funcs | scFv |
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