CytoStream™ Rabbit Anti-GPER1 Polyclonal Antibody (VS13-YC460) (CAT#: VS13-YC460)
The CytoStream™ Rabbit Anti-GPER1 Polyclonal Antibody is specific for human, mouse and rat GPER1. GPER1 encodes a membrane multi-pass protein in ER, associated with estrogen-induced signaling, impacting biological processes like bone development and fertility through non-genomic pathways. The anti-GPER1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 µg per test).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli, cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16718/1954_C1_62_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli, cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16718/1954_C1_62_selected.jpg
Figure 2 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/ihc_selected.jpg
(Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_B_9_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_8_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_5_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_5_6.jpg
Figure 8 Placenta
(Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_2_7.jpg
(Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_2_7.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27052/56949_A_7_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (AN3-CA, Daudi, fHDF/TERT166, Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164850-GPER1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164850-GPER1
Specifications
- Immunogen
- Recombinant human GPR30 (aa 251-375) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GPER1
- Species Reactivity
- Human, Mouse, Rat, Chicken (Predicted), Cow (Predicted), Horse (Predicted), Rabbit (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 µg per test
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- G Protein-Coupled Estrogen Receptor 1; Flow-Induced Endothelial G-Protein Coupled Receptor 1; Lymphocyte-Derived G-Protein Coupled Receptor; Chemoattractant Receptor-Like 2; G Protein-Coupled Receptor 30; Membrane Estrogen Receptor; IL8-Related Receptor DRY12; GPCR-Br; CMKRL2; LyGPR; GPR30; DRY12;
- Gene ID
- 2852
- UniProt ID
- Q99527
- Long Name
- G Protein-Coupled Estrogen Receptor 1
- Cellular Localization
- Cell membrane; Endoplasmic reticulum membrane; Golgi apparatus membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-415 | Recombinant Anti-human GPER1 Antibody | WB, IP, FuncS | IgG |
| MHH-415 | Recombinant Human Anti-human GPER1 Antibody | WB, Neut, FuncS | IgG |
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