Anti-GPI Immunohistochemistry Kit
CAT#: VS-0525-XY2887
The GPI IHC kit is optimized for high-fidelity staining of GPI in tissue samples. It works effectively with both paraffin and frozen tissue sections, ensuring reproducibility and high sensitivity. The optimized protocol guarantees high specificity with minimal background interference.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/285_G6_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18655/47250_A_6_6_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/286_G6_3_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/284_G6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105220-GPI
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- GPI
- Primary Antibody
- Rabbit Anti-GPI Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| ZG-0392J | Mouse Anti-GPI Recombinant Antibody (ZG-0392J) | WB, IHC, IF, ELISA | Mouse IgG |
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| VS7-HM1017 | Mouse Anti-GPI Recombinant Antibody (clone CBL327HM) | WB, IHC-p, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1491 | Rabbit Anti-GPI Recombinant Antibody (clone DS1491AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC461 | CytoStream™ Rabbit Anti-GPI Recombinant Antibody (VS13-YC461) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2888 | Anti-Mouse GPI Immunohistochemistry Kit | IHC |
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