Mouse Anti-GPI Recombinant Antibody (ZG-0392J)
CAT#: ZG-0392J
This product is a mouse antibody that recognizes GPI.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-GPI Recombinant Antibody (ZG-0392J) in WB
Western Blot analysis using GPI Monoclonal Antibody against HepG2 (1), SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).
IHC
Figure 2 Mouse Anti-GPI Recombinant Antibody (ZG-0392J) in IHC
Immunohistochemistry analysis of paraffin-embedded human Kidney tissues with AEC staining using GPI Monoclonal Antibody.
IF
Figure 3 Mouse Anti-GPI Recombinant Antibody (ZG-0392J) in IF
Confocal immunofluorescence analysis of L-02 cells using GPI Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/285_G6_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18655/47250_A_6_6_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/286_G6_3_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/284_G6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105220-GPI
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Specifications
- Immunogen
- Purified recombinant fragment of human GPI expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GPI
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; glucose-6-phosphate isomerase; autocrine motility factor; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen-36
- Gene ID
- 2821
- UniProt ID
- P06744
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| MOB-1303z | Mouse Anti-GPI Recombinant Antibody (clone 22C2) | ELISA, ICC, IF, IHC, WB | Mouse IgG1 |
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| VS3-WK773 | Mouse Anti-GPI Recombinant Antibody (clone PG002) | ELISA, CLIA, RIA, IHC, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1491 | Rabbit Anti-GPI Recombinant Antibody (clone DS1491AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC461 | CytoStream™ Rabbit Anti-GPI Recombinant Antibody (VS13-YC461) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2887 | Anti-GPI Immunohistochemistry Kit | IHC | |
| VS-0525-XY2888 | Anti-Mouse GPI Immunohistochemistry Kit | IHC |
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