Rabbit Anti-GPX4 Recombinant Antibody (clone 9D4)
CAT#: ZG-0692J
This product is a rabbit antibody that recognizes GPX4.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-GPX4 Antibody (ZG-0692J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58546/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167468-GPX4
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Specifications
- Immunogen
- A synthesized peptide derived from human GPX4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GPX4
- Species Reactivity
- Human
- Clone
- 9D4
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
- Alternative Names
- MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx; phospholipid hydroperoxide glutathione peroxidase; epididymis secretory sperm binding protein; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; sperm nucleus glutathione peroxidase
- Gene ID
- 2879
- UniProt ID
- P36969
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GPX4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1499 | Rabbit Anti-GPX4 Recombinant Antibody (clone DS1499AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0688-CN | Rabbit Anti-GPX4 Recombinant Antibody (clone JU11-31) | WB, IHC | Rabbit IgG |
| MRO-1906-CN | Rabbit Anti-GPX4 Polyclonal Antibody (MRO-1906-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-0367JF | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1) | WB, IHC, IF | Mouse IgG2b, κ |
| VS3-FY613 | Recombinant Rabbit Anti-GPX4 Antibody (clone R03-6E6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-XY3371 | Rabbit Anti-GPX4 Recombinant Antibody (clone R01-6I5) | WB, IHC-F, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-F(E) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); Fab Fragment | IB, IP, IHC, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-S(P) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); scFv Fragment | IB, IP, IHC, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2900 | Anti-GPX4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2901 | Anti-Mouse GPX4 Immunohistochemistry Kit | IHC |
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