Mouse Anti-GPx4 Recombinant Antibody (clone 63-1)
CAT#: FAMAB-0367JF
Recombinant monoclonal antibody specially binds to human and mouse GPx4. 63-1 is a mouse monoclonal antibody that can be used to quantify enzyme expression by immunoblotting, immunohistochemistry and immunoprecipitation.
Published Data
Gene Expression
IF
Figure 1 GPX4 in nigral inclusions of PD brain.
The above example shows some co-localization of GPX4 with AS within a Lewy body, while the below example shows GPX4 only around the Lewy body perimeter. Scale bars: A, 20 μm, B, 10 μm.
Bellinger, F. P., Bellinger, M. T., Seale, L. A., Takemoto, A. S., Raman, A. V., Miki, T., ... & Ross, G. W. (2011). Glutathione peroxidase 4 is associated with neuromelanin in substantia nigra and dystrophic axons in putamen of Parkinson's brain. Molecular neurodegeneration, 6(1), 1-10.
IHC
Figure 2 GPX4 expression in putamen.
GPX4 immunoreactivity is detectable in cell bodies in the putamen of control brains (white arrowheads).
Bellinger, F. P., Bellinger, M. T., Seale, L. A., Takemoto, A. S., Raman, A. V., Miki, T., ... & Ross, G. W. (2011). Glutathione peroxidase 4 is associated with neuromelanin in substantia nigra and dystrophic axons in putamen of Parkinson's brain. Molecular neurodegeneration, 6(1), 1-10.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58546/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167468-GPX4
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Specifications
- Immunogen
- Recombinant human cGPx4
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human GPx, Mouse GPx4
- Species Reactivity
- Human
- Clone
- 63-1
- Applications
- WB, IHC, IF
- Related Disease
- Oxidative defense system damage
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The GPx4 antibody has been reported in applications of Western Blot, Immunohistochemistry, Immunofluorescence.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0762MZ | Recombinant Mouse Anti-Human GPX4 Antibody (clone 8B5) | ELISA, IP, WB | Mouse antibody |
| MRO-0688-CN | Rabbit Anti-GPX4 Recombinant Antibody (clone JU11-31) | WB, IHC | Rabbit IgG |
| MRO-1906-CN | Rabbit Anti-GPX4 Polyclonal Antibody (MRO-1906-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY613 | Recombinant Rabbit Anti-GPX4 Antibody (clone R03-6E6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS13-YC464 | Rabbit Anti-GPX4 Recombinant Antibody (VS13-YC464) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1499 | Rabbit Anti-GPX4 Recombinant Antibody (clone DS1499AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-F(E) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); Fab Fragment | IB, IP, IHC, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-S(P) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); scFv Fragment | IB, IP, IHC, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2900 | Anti-GPX4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2901 | Anti-Mouse GPX4 Immunohistochemistry Kit | IHC |
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