Mouse Anti-GPx4 Recombinant Antibody (clone 63-1) (CAT#: FAMAB-0367JF)
Recombinant monoclonal antibody specially binds to human and mouse GPx4. 63-1 is a mouse monoclonal antibody that can be used to quantify enzyme expression by immunoblotting, immunohistochemistry and immunoprecipitation.
Specific Inquiry
IF
Figure 1 GPX4 in nigral inclusions of PD brain.
The above example shows some co-localization of GPX4 with AS within a Lewy body, while the below example shows GPX4 only around the Lewy body perimeter. Scale bars: A, 20 μm, B, 10 μm.
Bellinger, F. P., Bellinger, M. T., Seale, L. A., Takemoto, A. S., Raman, A. V., Miki, T., ... & Ross, G. W. (2011). Glutathione peroxidase 4 is associated with neuromelanin in substantia nigra and dystrophic axons in putamen of Parkinson's brain. Molecular neurodegeneration, 6(1), 1-10.
IHC
Figure 2 GPX4 expression in putamen.
GPX4 immunoreactivity is detectable in cell bodies in the putamen of control brains (white arrowheads).
Bellinger, F. P., Bellinger, M. T., Seale, L. A., Takemoto, A. S., Raman, A. V., Miki, T., ... & Ross, G. W. (2011). Glutathione peroxidase 4 is associated with neuromelanin in substantia nigra and dystrophic axons in putamen of Parkinson's brain. Molecular neurodegeneration, 6(1), 1-10.
WB
Figure 3 Confirmation of GPX4 binding to active (1S, 3R)-RSL3 affinity probe.
The purified protein samples were probed for GPX4 by western blot using GPX4-specific antibody.
Yang, W. S., SriRamaratnam, R., Welsch, M. E., Shimada, K., Skouta, R., Viswanathan, V. S., ... & Brown, L. M. (2014). Regulation of ferroptotic cancer cell death by GPX4. Cell, 156(1-2), 317-331.
Specifications
- Immunogen
- Recombinant human cGPx4
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human GPx, Mouse GPx4
- Species Reactivity
- Human
- Clone
- 63-1
- Applications
- WB, IHC, IF
- Related Disease
- Oxidative defense system damage
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The GPx4 antibody has been reported in applications of Western Blot, Immunohistochemistry, Immunofluorescence.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 63-1"
See other products for "GPX4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0762MZ | Recombinant Mouse Anti-Human GPX4 Antibody (clone 8B5) | ELISA, IP, WB | Mouse antibody |
| MRO-0688-CN | Rabbit Anti-GPX4 Recombinant Antibody (clone JU11-31) | WB, IHC | Rabbit IgG |
| MRO-1906-CN | Rabbit Anti-GPX4 Polyclonal Antibody (MRO-1906-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0692J | Rabbit Anti-GPX4 Recombinant Antibody (clone 9D4) | ELISA, IHC | Rabbit IgG |
| VS3-FY613 | Recombinant Rabbit Anti-GPX4 Antibody (clone R03-6E6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1499 | Rabbit Anti-GPX4 Recombinant Antibody (clone DS1499AB) | WB, IHC, ICC, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-F(E) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); Fab Fragment | IB, IP, IHC, ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-S(P) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); scFv Fragment | IB, IP, IHC, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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