Rabbit Anti-GRIA1 Recombinant Antibody (clone R01-3U6) (CAT#: VS3-XY3373)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat GRIA1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R01-3U6

• Applications
• WB, IHC-P, IP

• Related Disease
• Intellectual Developmental Disorder, Autosomal Recessive 76

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100
  IP: 1/20

• Alternative Names
• GLUH1; GLUR1; GLURA; GluA1; HBGR1; MRD67; MRT76

• Gene ID
• 2890

• UniProt ID
• P42261

• Long Name
• Glutamate ionotropic receptor AMPA type subunit 1

• Sequence Similarities
• Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

• Cellular Localization
• Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane, Postsynaptic cell membrane, Synapse

• Post Translation Modifications
• Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
  Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity).
  Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).

• Protein Refseq
• NP_000818.2; NP_001107655.1; NP_001244948.1

• Function
• Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody