Mouse Anti-GTF2B Monoclonal Antibody (clone 4C1) (CAT#: VS7-0425-WR3)
This product is a mouse IgG1 antibody that binds to Human GTF2B. 4C1 recognizes human GTF2B and can be used for WB assay. The antibody also cross reacts with monkey.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/1153_H2_2_selected.jpg

(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/ihc_selected.jpg

(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_B_9_5.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_3.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_8_5.jpg

(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_4_6.jpg

(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_8.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137947-GTF2B
Specifications
- Immunogen
- Recombinant human GTF2B fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human and Monkey GTF2B
- Species Reactivity
- Human, Monkey
- Clone
- 4C1
- Molecule Class
- Transcription Factor
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Spinocerebellar ataxia 17; Cockayne syndrome; Xeroderma pigmentosum.
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- GTF2B; TF2B; TFIIB; general transcription factor IIB; transcription factor II B
- Gene ID
- 2959
- UniProt ID
- Q00403
- Long Name
- General transcription factor IIB
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Phosphorylation
- Protein Refseq
- NP_001505
- Function
- General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329).; Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312).; Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).; Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).; Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329).; Modulates transcription start site selection (PubMed:10318856).; Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "GTF2B"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2012CT | Recombinant Mouse anti-Human GTF2B Monoclonal antibody (JJC2) | ChIP, IP, WB | |
VS3-QX489 | Mouse Anti-GTF2B Recombinant Antibody (clone 2F6-A3) | WB | Mouse IgG2b |
VS3-CJ27 | Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27) | WB, ELISA | Mouse IgG |
VS13-YC484 | Rabbit Anti-GTF2B Recombinant Antibody (VS13-YC484) | WB, IP | Rabbit IgG |
VS7-0425-WR4 | Mouse Anti-TFIIB Recombinant Antibody (VS7-0425-WR4) | WB, IHC-P, IF, ICC | Mouse IgG |
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