Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27) (CAT#: VS3-CJ27)

• Immunogen
• Recombinant full length of human GTF2B

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human GTF2B

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:500-1:1000).

• Alternative Names
• TF2B; TFIIB

• Gene ID
• 2959

• UniProt ID
• Q00403

• Sequence Similarities
• Belongs to the TFIIB family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Acetylated (PubMed:24441171).
  Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).

• Protein Refseq
• NP_001505.1

• Function
• General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).
  Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
  Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
  Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
  Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
  Modulates transcription start site selection (PubMed:10318856).
  Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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