Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27)
CAT#: VS3-CJ27
This product is a mouse antibody that recognizes GTF2B.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/1153_H2_2_selected.jpg
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Specifications
- Immunogen
- Recombinant full length of human GTF2B
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GTF2B
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:500-1:1000).
Target
- Alternative Names
- TF2B; TFIIB
- Gene ID
- 2959
- UniProt ID
- Q00403
- Sequence Similarities
- Belongs to the TFIIB family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Acetylated (PubMed:24441171).
Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).
- Protein Refseq
- NP_001505.1
- Function
- General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).
Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
Modulates transcription start site selection (PubMed:10318856).
Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
See other products for "GTF2B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2012CT | Recombinant Mouse anti-Human GTF2B Monoclonal antibody (JJC2) | ChIP, IP, WB | |
| VS3-QX489 | Mouse Anti-GTF2B Recombinant Antibody (clone 2F6-A3) | WB | Mouse IgG2b |
| VS4-WK206 | Mouse Anti-GTF2B Recombinant Antibody (VS4-WK206) | ELISA, WB, IHC, ICC | Mouse IgG |
| VS13-YC484 | Rabbit Anti-GTF2B Recombinant Antibody (VS13-YC484) | WB, IP | Rabbit IgG |
| VS7-0425-WR3 | Mouse Anti-GTF2B Monoclonal Antibody (clone 4C1) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1539 | Rabbit Anti-GTF2B Recombinant Antibody (clone DS1539AB) | WB, IHC, IP, FC | Rabbit IgG |
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