Rabbit Anti-H1-4 (pT17) Recombinant Antibody (clone R07-3C1) (CAT#: VS3-WK2116)
This product is a rabbit recombinant antibody that is specific to phosphorylated H1-4. The target of this antibody is phospho-H1-4 (Thr17). The clone R07-3C1 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IHC staining at a dilution of 1/50-1/100. The molecular weight of H1-4 (pThr17) observed in WB experiments using this antibody is 30 kDa.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/if_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55907/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55907/ihc_selected.jpg
Figure 3 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_B_9_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_3.jpg
Figure 6 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_7_4.jpg
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_7_4.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_6_6.jpg
Figure 9 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_B_5_4.jpg
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_B_5_4.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11506/33405_A_9_8.jpg
Figure 11 RNA cell line category: Cell line enhanced (RPMI-8226, U-266/70, U-266/84, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168298-H1-4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168298-H1-4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr17 of human, mouse and rat H1-4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3C1
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5
- Gene ID
- 3008
- UniProt ID
- P10412
- Long Name
- H1.4 linker histone, cluster member
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0714J | Rabbit Anti-H1-4 Recombinant Antibody (clone 3E1) | ELISA, IHC, IF | Rabbit IgG |
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