Rabbit Anti-H1-4 Recombinant Antibody (clone 3E1) (CAT#: ZG-0714J)
This product is a rabbit antibody that recognizes H1-4.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 2 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IF
Immunofluorescence staining of MCF-7 cells with this product at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H1-4
- Species Reactivity
- Human
- Clone
- 3E1
- Applications
- ELISA, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
IHC:1:50-1:500, IF:1:30-1:200
Target
- Alternative Names
- H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5; histone H1.4; H1 histone family, member 4; histone 1, H1e; histone H1b; histone H1s-4; histone cluster 1 H1 family member e; histone cluster 1, H1e
- Gene ID
- 3008
- UniProt ID
- P10412
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2116 | Rabbit Anti-H1-4 (pT17) Recombinant Antibody (clone R07-3C1) | WB, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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