Rabbit Anti-H1-4 Recombinant Antibody (clone 3E1) (CAT#: ZG-0714J)
This product is a rabbit antibody that recognizes H1-4.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 2 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-H1-4 Antibody (ZG-0714J) in IF
Immunofluorescence staining of MCF-7 cells with this product at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H1-4
- Species Reactivity
- Human
- Clone
- 3E1
- Applications
- ELISA, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
IHC:1:50-1:500, IF:1:30-1:200
Target
- Alternative Names
- H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5; histone H1.4; H1 histone family, member 4; histone 1, H1e; histone H1b; histone H1s-4; histone cluster 1 H1 family member e; histone cluster 1, H1e
- Gene ID
- 3008
- UniProt ID
- P10412
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2116 | Rabbit Anti-H1-4 (pT17) Recombinant Antibody (clone R07-3C1) | WB, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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