Mouse Anti-H3K9ac Recombinant Antibody (clone scFv-58F)
CAT#: VS-0623-WK91
The antibody clone scFv-58F was produced by immunizing mouse with H3K9ac. It can be used for detection of human H3K9ac in ELISA, WB, IF and IP assays.
Published Data
DB
Figure 1 Binding profile of scFv-58F to an annotated dot-blot array of 384 modified histone peptides.
Two experimental replicas are shown. The spot in the lower right part of the filter is a positive control (c-myc).
IF
Figure 2 Immunofluorescence of HeLa cell nuclei using ScFv-58F-HA, overlaid with Lamin staining.
IP
Figure 3 IP of chromatin solutions from yeast cells intracellularly expressing either the unrelated ScFv-#2-VP16 or ScFv-58F-HA, blotted for AcK9H3.
FuncS
Figure 4 Validation by real-time PCR of selected differentially expressed genes between ScFv58F and ScFv112A samples.
Among the most significant hits we found were PHO84 and PHO89 (involved in phosphate transport and metabolism), which are known to be strongly regulated by histone acetylation levels in yeast.
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Specifications
- Immunogen
- H3K9ac
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human H3K9ac
- Species Reactivity
- Human
- Clone
- scFv-58F
- Applications
- ELISA, DB, WB, IF, IP, FuncS
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 100 ng/µL
Target
- Alternative Names
- Potassium Voltage-Gated Channel Subfamily A Member 1; Potassium Voltage-Gated Channel; Shaker-Related Subfamily; Member 1 (Episodic Ataxia With Myokymia); Voltage-Gated Potassium Channel Subunit Kv1.1; Voltage-Gated Potassium Channel HBK1; Voltage-Gated K(+) Channel HuKI; Potassium Channel; Voltage Gated Shaker Related Subfamily A; Member 1; KV1.1; HBK1
- Long Name
- H3K9ac
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "H3K9ac"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0146YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v1) | IF | Mouse IgG |
| FAMAB-0147YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v2) | IF | Mouse IgG |
| FAMAB-0148YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v3) | IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0146-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v1); scFv Fragment | IF | Mouse scFv |
| FAMAB-0147-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v2); scFv Fragment | IF | Mouse scFv |
| FAMAB-0148-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v3); scFv Fragment | IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0146-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v1); Fab Fragment | IF | Mouse Fab |
| FAMAB-0147-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v2); Fab Fragment | IF | Mouse Fab |
| FAMAB-0148-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v3); Fab Fragment | IF | Mouse Fab |
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