The antibody clone scFv-58F was produced by immunizing mouse with H3K9ac. It can be used for detection of human H3K9ac in ELISA, WB, IF and IP assays.
Figure 1 Binding profile of scFv-58F to an annotated dot-blot array of 384 modified histone peptides.
Two experimental replicas are shown. The spot in the lower right part of the filter is a positive control (c-myc).
Figure 2 Immunofluorescence of HeLa cell nuclei using ScFv-58F-HA, overlaid with Lamin staining.
Figure 3 IP of chromatin solutions from yeast cells intracellularly expressing either the unrelated ScFv-#2-VP16 or ScFv-58F-HA, blotted for AcK9H3.
Figure 4 Validation by real-time PCR of selected differentially expressed genes between ScFv58F and ScFv112A samples.
Among the most significant hits we found were PHO84 and PHO89 (involved in phosphate transport and metabolism), which are known to be strongly regulated by histone acetylation levels in yeast.
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• Confirmed specificity
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• Excellent batch-to-batch consistency
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CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0146YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v1) | IF | Mouse IgG |
FAMAB-0147YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v2) | IF | Mouse IgG |
FAMAB-0148YC | Mouse Anti-H3K9ac Recombinant Antibody (clone v3) | IF | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0146-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v1); scFv Fragment | IF | Mouse scFv |
FAMAB-0147-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v2); scFv Fragment | IF | Mouse scFv |
FAMAB-0148-YC-S(P) | Mouse Anti-H3K9ac Recombinant Antibody (clone v3); scFv Fragment | IF | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0146-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v1); Fab Fragment | IF | Mouse Fab |
FAMAB-0147-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v2); Fab Fragment | IF | Mouse Fab |
FAMAB-0148-YC-F(E) | Mouse Anti-H3K9ac Recombinant Antibody (clone v3); Fab Fragment | IF | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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