Mouse Anti-HAO1 Antibody (clone Mix) (CAT#: ZG-0093C)

This product is a mouse antibody that recognizes HAO1. It can be used in a wide variety of applications, such as: WB, IHC-P, IF/ICC.


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  • Tested Data
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IF

Figure 1 Mouse Anti-HAO1 Antibody in IF

Figure 1 Mouse Anti-HAO1 Antibody in IF

Immunofluorescence analysis of Hela cell. 1, Chk2 (phospho Thr68) Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HAO1 Monoclonal Antibody (Mix) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).

IHC

Figure 2 Mouse Anti-HAO1 Antibody in IHC

Figure 2 Mouse Anti-HAO1 Antibody in IHC

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, HAO1 Monoclonal Antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IHC

Figure 3 Mouse Anti-HAO1 Antibody in IHC

Figure 3 Mouse Anti-HAO1 Antibody in IHC

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, HAO1 Monoclonal Antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IHC

Figure 4 Mouse Anti-HAO1 Antibody in IHC

Figure 4 Mouse Anti-HAO1 Antibody in IHC

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, HAO1 Monoclonal Antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IF

Figure 5 Mouse Anti-HAO1 Antibody in IF

Figure 5 Mouse Anti-HAO1 Antibody in IF

Immunofluorescence analysis of Human-appendix tissue. 1, HAO1 Monoclonal Antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IF

Figure 6 Mouse Anti-HAO1 Antibody in IF

Figure 6 Mouse Anti-HAO1 Antibody in IF

Immunofluorescence analysis of Mouse-spleen tissue. 1, HAO1 Monoclonal Antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

WB

Figure 7 Mouse Anti-HAO1 Antibody in WB

Figure 7 Mouse Anti-HAO1 Antibody in WB

WB analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody .


Specifications

  • Immunogen
  • Recombinant Protein of HAO1
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • HAO1
  • Species Reactivity
  • Mouse, Rat
  • Clone
  • Mix
  • Applications
  • WB, IHC-P, IF/ICC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Storage
  • Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.

Target

  • Alternative Names
  • Hydroxyacid Oxidase 1; Hydroxyacid Oxidase (Glycolate Oxidase) 1; Glycolate Oxidase 1; HAOX1; GOX1; GOX; (S)-2-Hydroxy-Acid Oxidase; Glycolate Oxidase; EC 1.1.3.15

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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