AbPlus™ Anti-HDGF Magnetic Beads (2F12)
CAT#: VS-0424-XY121
The AbPlus Anti-HDGF Magnetic Beads (2F12) is an innovative affinity resin which is bound with anti-HDGF specific antibody (2F12). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of HDGF protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53422/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26035/if_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143321-HDGF
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-HDGF specific antibody (2F12)
- Target
- HDGF
- Immunogen
- Hepatoma derived growth factor
- Target Species
- Human
- Antibody Clone
- 2F12
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
- Alternative Names
- HMG1L2
- Gene ID
- 3068
- UniProt ID
- P51858
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "HDGF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2467z | Mouse Anti-HDGF Recombinant Antibody (clone 33H7) | ELISA, ICC, IF, IHC, WB | Mouse IgG2a |
| MOB-0146CT | Recombinant Mouse anti-Human HDGF Monoclonal antibody (37D5b) | Dot, WB | |
| HPAB-0032-LSX | Mouse Anti-HDGF Recombinant Antibody (clone 2F12) | ELISA, WB, IP, IF, ICC | Mouse IgG1, κ |
| HPAB-0033-LSX | Mouse Anti-HDGF Recombinant Antibody (clone H3) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| VS3-FY2147 | Rabbit Anti-HDGF Recombinant Antibody (clone R78-2C-3) | WB, IHC-P, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1581 | Rabbit Anti-HDGF Recombinant Antibody (clone DS1581AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); scFv Fragment | ELISA, WB, IP, IF, ICC | Mouse scFv |
| HPAB-0033-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone H3); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| HPAB-0034-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); Fab Fragment | ELISA, WB, IP, IF, ICC | Mouse Fab |
| HPAB-0033-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone H3); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| HPAB-0034-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1007 | Anti-HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3107 | Anti-Mouse HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3106 | Anti-Human HDGF Immunohistochemistry Kit | IHC |
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