Mouse Anti-HDGF Recombinant Antibody (clone H3)
CAT#: HPAB-0033-LSX
Mouse antibody H3 provides methods of cancer therapy or diagnosis involving targeting hepatoma-derived growth factor (HDGF). The antibody may be used to inhibit HDGF, optionally coupled to or combined with other cancer therapies.
Published Data
Gene Expression
WB
Figure 1 Western blot analysis testing various monoclonal antibodies developed to against HDGF (lysate derived from H1944 cells).
Ren, H., Chu, Z., & Mao, L. (2009). Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Molecular cancer therapeutics, 8(5), 1106-1112.
IHC
Figure 2 Distribution ofthe antibodies in tumor tissue sections (detected by using specific antimouse IgG).
Antibodies used were labeled on the side of each section for easy reference.
Ren, H., Chu, Z., & Mao, L. (2009). Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Molecular cancer therapeutics, 8(5), 1106-1112.
FuncS
Figure 3 Growth curves of tumors treated with various antibodies.
Ren, H., Chu, Z., & Mao, L. (2009). Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Molecular cancer therapeutics, 8(5), 1106-1112.
FuncS
Figure 4 Tumor weight measured for each treatment groups. P values are based on comparisons with M31 control group.
Ren, H., Chu, Z., & Mao, L. (2009). Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Molecular cancer therapeutics, 8(5), 1106-1112.
FuncS
Figure 5 Ki-67 expression in H3-treated tumors
Ren, H., Chu, Z., & Mao, L. (2009). Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Molecular cancer therapeutics, 8(5), 1106-1112.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53422/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26035/if_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143321-HDGF
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Specifications
- Immunogen
- HDGF
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse HDGF
- Species Reactivity
- Human, Mouse
- Clone
- H3
- Applications
- ELISA, WB, RIA, FC, ICC, IHC, FuncS
- Related Disease
- Lung cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Sections (4 Mm) were from formalin-fixed and paraffin-embedded tissue blocks or OCT-embedded frozen tissues. All the sections were mounted on positively charged glass slides. For formalin-fixed tissues, sections were deparaffinized and stained with appropriate antibody using ABC Elite system. For frozen tissues, the sections were fixed with acetone before being processed for staining. Diaminobenzidine was used as a chromogen and commercial hematoxylin was used for counterstaining. For microvessel density analysis, CD31 staining was measured using ×10 objective magnification for three to six randomly selected fields (2.18 mm2 per field). Each field was then divided into 155 squares (grids). The grids with CD31 staining was counted as positive and total positive grids divided by the total grids measured was used to calculate percentage of positive grids for each sample.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2467z | Mouse Anti-HDGF Recombinant Antibody (clone 33H7) | ELISA, ICC, IF, IHC, WB | Mouse IgG2a |
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| HPAB-0032-LSX | Mouse Anti-HDGF Recombinant Antibody (clone 2F12) | ELISA, WB, IP, IF, ICC | Mouse IgG1, κ |
| HPAB-0034-LSX | Mouse Anti-HDGF Recombinant Antibody (clone C1) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| VS3-FY2147 | Rabbit Anti-HDGF Recombinant Antibody (clone R78-2C-3) | WB, IHC-P, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); scFv Fragment | ELISA, WB, IP, IF, ICC | Mouse scFv |
| HPAB-0033-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone H3); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| HPAB-0034-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); Fab Fragment | ELISA, WB, IP, IF, ICC | Mouse Fab |
| HPAB-0033-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone H3); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| HPAB-0034-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0325-XY1007 | Anti-HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3107 | Anti-Mouse HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3106 | Anti-Human HDGF Immunohistochemistry Kit | IHC |
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