Mouse Anti-HDGF Recombinant Antibody (clone 2F12) (CAT#: HPAB-0032-LSX)
The specific monoclonal antibody of anti-hepatoma derived growth factor (HDGF) can be used to make HDGF detection reagent suitable for a plurality of detection method.
Specific Inquiry
WB, IP
Figure 1 Characterization of anti-HDGF antibodies by western blotting and immunoprecipitation.
A) Western blotting: lane 1, pre-immune mouse serum; lane 2, mouse anti-His mAb; lane 3, anti-HDGF mAb 2F12; lane 4, final boost mouse serum. B) mAb 2F12 immunoprecipitation: lane 1, negative control (protein G beads+ 2F12); lane 2, positive control (0.2 µg His-HDGF); lane 3, NIH3T3 cell lysate; lane 4, Hep-G2 cell lysate; lane 5, M14 cell lysate.
Zhang, A., Long, W., Guo, Z., Liu, G., Hu, Z., Huang, Y.,... & Everett, A. D. (2010). Development and clinical evaluation of a multi-purpose mAb and a sandwich ELISA test for hepatoma-derived growth factor in lung cancer patients. Journal of immunological methods, 355(1-2), 61-67.
IF
Figure 2 Immunofluorescent staining of anti-HDGF antibodies (400×).
HDGF-positive Hep-G2 cells and HDGF-negative NIH3T3 cells were co-cultured and fixed with 10% formalin at RT for 30 min. A) Rabbit anti-HDGF pAb (FITC conjugate); B) Nomarski; C) Anti-HDGF mAb 2F12 (Rhodmine red conjugate); D) DAPI staining; E) Overlap.
Zhang, A., Long, W., Guo, Z., Liu, G., Hu, Z., Huang, Y.,... & Everett, A. D. (2010). Development and clinical evaluation of a multi-purpose mAb and a sandwich ELISA test for hepatoma-derived growth factor in lung cancer patients. Journal of immunological methods, 355(1-2), 61-67.
IHC
Figure 3 HDGF immunohistochemical staining of cell arrays (400×).
HDGF-positive staining was found on tumor cell lines of glioblastoma (U87, U118, U373, and SW1873); prostate cancer (PC-3); leiomyosarcoma (SKLMS-1); breast cancer (MCF-7); and melanoma (M14). One glioblastoma cell line (DBTRG) was HDGFnegative. NIH3T3 cells were used as a negative control.
Zhang, A., Long, W., Guo, Z., Liu, G., Hu, Z., Huang, Y.,... & Everett, A. D. (2010). Development and clinical evaluation of a multi-purpose mAb and a sandwich ELISA test for hepatoma-derived growth factor in lung cancer patients. Journal of immunological methods, 355(1-2), 61-67.
IHC
Figure 4 HDGF immunohistochemical staining of breast cancer (100×).
A) HDGF mainly localized in the nucleus; B) HDGF in both nucleus and cytoplasm; C) weak staining of fewer cells in adjacent normal tissue; D) only tumor cells are positive in local lymph node metastasis.
Zhang, A., Long, W., Guo, Z., Liu, G., Hu, Z., Huang, Y.,... & Everett, A. D. (2010). Development and clinical evaluation of a multi-purpose mAb and a sandwich ELISA test for hepatoma-derived growth factor in lung cancer patients. Journal of immunological methods, 355(1-2), 61-67.
IHC
Figure 5 HDGF immunohistochemical staining of glioma (100×).
A to D represent clinical stages I; I-II; III, and IV, respectively, showing the positive correlation between HDGF expression level and clinical stage.
Zhang, A., Long, W., Guo, Z., Liu, G., Hu, Z., Huang, Y.,... & Everett, A. D. (2010). Development and clinical evaluation of a multi-purpose mAb and a sandwich ELISA test for hepatoma-derived growth factor in lung cancer patients. Journal of immunological methods, 355(1-2), 61-67.
Specifications
- Immunogen
- Hepatoma derived growth factor (HDGF)
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human HDGF
- Species Reactivity
- Human
- Clone
- 2F12
- Applications
- ELISA, WB, IP, IF, ICC
- Related Disease
- Breast cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunoprecipitation, Immunohistochemistry (1:2000), Immunofluorescence (1:1000).
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2467z | Mouse Anti-HDGF Recombinant Antibody (clone 33H7) | ELISA, ICC, IF, IHC, WB | Mouse IgG2a |
| MOB-0146CT | Recombinant Mouse anti-Human HDGF Monoclonal antibody (37D5b) | Dot, WB | |
| HPAB-0033-LSX | Mouse Anti-HDGF Recombinant Antibody (clone H3) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| HPAB-0034-LSX | Mouse Anti-HDGF Recombinant Antibody (clone C1) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| VS3-FY2147 | Rabbit Anti-HDGF Recombinant Antibody (clone R78-2C-3) | WB, IHC-P, IF, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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