Mouse Anti-His Tag Recombinant Antibody (clone 6A8H8)

CAT#: VS7-0425-WR36

This product is a mouse IgG1 recombinant antibody that binds to His Tag. The antibody 1H3E12 recognizes a specific amino acid domains: HHHHHHHH-KLH. It can be used for WB and IP assays.

Specifications

  • Immunogen
  • Recombinant His Tag (AA: HHHHHHHH-KLH) fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • His Tag
  • Species Reactivity
  • Tag
  • Clone
  • 6A8H8
  • Molecule Class
  • Tag
  • Applications
  • WB, IP
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:1000
    Immunohistochemistry-Paraffin: 1:100 - 1:1000
    Immunocytochemistry: 1:100 - 1:1000
    Flow Cytometry: 1:200 - 1:400
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • 6 His tag; HHHHHH tag; polyhistidine-tag; hexa histidine-tag; 6xHis-tag; His6 tag.
  • Long Name
  • Polyhistidine-tag
  • Function
  • Tag protein for protein purification, binding assays, fluorescent tags, and Fluorohistidine tags.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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