CytoStream™ Rabbit Anti-His tag Recombinant Antibody (VS13-YC506)
CAT#: VS13-YC506
The CytoStream™ Rabbit Anti-His tag Recombinant Antibody is specific for His tag. His tag is a polyhistidine tag used in protein purification, typically containing six histidine residues to facilitate binding purification methods. The anti-His tag antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/200-1/500).
Specifications
- Immunogen
- Synthetic peptide: HHHHHHHHC.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- His tag
- Species Reactivity
- Species independent
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/5000
Immunofluorescence (IF-cell): 1/5000
Immunohistochemistry-paraffin (IHC-P): 1/5000
Flow cytometry (FC): 1/200-1/500
Immunoprecipitation (IP): 1-2 μg per 500 μL of cell lysate
Target
- Alternative Names
- polyhistidine tag
- Long Name
- polyhistidine tag
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "His tag"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-134 | Mouse Anti-His Tag Recombinant Antibody (clone YC103); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| HPAB-0328-WJ-S(P) | Mouse Anti-His Tag Recombinant Antibody; scFv Fragment (HPAB-0328-WJ-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-M0257-YC-S(P) | Mouse Anti-HIS Tag Recombinant Antibody (clone 4C9); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-134 | Mouse Anti-His Tag Recombinant Antibody (clone YC103); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0328-WJ-F(E) | Mouse Anti-His Tag Recombinant Antibody; Fab Fragment (HPAB-0328-WJ-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-M0257-YC-F(E) | Mouse Anti-HIS Tag Recombinant Antibody (clone 4C9); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0707MZ | Chicken Anti-His tag Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ANTIHIS-200 | Mouse Anti-His Tag Recombinant Antibody (clone YC102) | ELISA, WB, FC, IHC, IF, IP | Mouse IgG1, κ |
| MOB-225CQ | Mouse Anti-His Tag Recombinant Antibody (clone AD1.1.10) | ELISA, WB | Mouse IgG |
| MRO-0014-CN | Mouse Anti-His Tag Recombinant Antibody (clone 8-F0) | WB | Mouse IgG2a |
| HPAB-0328-WJ | Mouse Anti-His Tag Recombinant Antibody (HPAB-0328-WJ) | ELISA, WB | Mouse IgG1, κ |
| VS-0723-WK311 | Rabbit Anti-His Tag Recombinant Antibody (VS-0723-WK311) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0388-YC | Recombinant Alpaca Anti-HIS Tag Single Domain Antibody (HPAB-M0388-YC) | ELISA | Alpaca VHH |
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