CytoStream™ Rabbit Anti-His tag Recombinant Antibody (VS13-YC506)

CAT#: VS13-YC506

The CytoStream™ Rabbit Anti-His tag Recombinant Antibody is specific for His tag. His tag is a polyhistidine tag used in protein purification, typically containing six histidine residues to facilitate binding purification methods. The anti-His tag antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/200-1/500).

Specifications

  • Immunogen
  • Synthetic peptide: HHHHHHHHC.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • His tag
  • Species Reactivity
  • Species independent
  • Conjugate
  • NONE

Product Property

  • Clonality
  • Monoclonal
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
  • Preservative
  • 0.05% Sodium azide
  • Storage
  • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Shipping with ice pack.

Applications

  • Application Notes
  • Western blot (WB): 1/5000
    Immunofluorescence (IF-cell): 1/5000
    Immunohistochemistry-paraffin (IHC-P): 1/5000
    Flow cytometry (FC): 1/200-1/500
    Immunoprecipitation (IP): 1-2 μg per 500 μL of cell lysate

Target

  • Alternative Names
  • polyhistidine tag
  • Long Name
  • polyhistidine tag
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

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BRD-0707MZ Chicken Anti-His tag Polyclonal IgY WB Chicken antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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