Mouse Anti-Histone H3 Recombinant Antibody (clone CBS173)

CAT#: MRO-0228YJ

This product is a monoclonal mouse antibody that recognizes Histone H3.

Specifications

  • Immunogen
  • Mouse monoclonal antibody is prepared by immunizing recombinant protein.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human Histone H3
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • CBS173
  • Applications
  • WB, IF, ICC, IP, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Purified
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Buffer
  • 1mg/ml in PBS, pH 7.4.
  • Storage
  • Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • WB: 1:1000-1:5000
    IF/ICC: 1:200
    IP: 1:200

Target

  • Alternative Names
  • H3 histone family, member A; H3/A; H31_HUMAN; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; Histone cluster 1, H3a; Histone H3. 1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3. 3A; HIST1 cluster, H3E; H3 histone family, member A; H3. 1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3. 1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3. 1; Histone H3. 2; Histone H3. 3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; H3 histone family 3A; H3 histone family 3B; H3 histone, family 3B (H3. 3B); H3. 3; H3. 3A; H3. 3B; H33_HUMAN; H3F3; H3F3A; H3f3b; Histone H3. 3; Histone H3. 3Q; Histone H3. A; Histone H3. B; MGC87782; MGC87783;
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Breast cancer biomarkers at key points during disease progression
Excellent Nuclear Staining
We used this monoclonal antibody for Immunocytochemistry to visualize chromatin structure. The nuclear signal was very strong and specific for Histone H3 in our human cell lines. It also worked well for mouse samples.
Breast cancer biomarkers at key points during disease progression
Versatile Antibody For Chromatin
This is a staple in our lab for Western Blot and Immunoprecipitation. It consistently detects the H3 core component of nucleosomes. We appreciate the versatility across different species like rat and mouse.

Q&As

  1. Can this detect H3 in rats?

    A: Yes, this monoclonal mouse antibody recognizes Histone H3 and is reactive with Human, Mouse, and Rat samples. It is suitable for multiple applications including Western Blot and Immunoprecipitation.

  2. What is the recommended storage?

    A: You should store the product at 4 degrees Celsius for short-term use. For long-term storage, we recommend aliquoting and storing at -20 degrees Celsius to avoid repeated freeze and thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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