Mouse Anti-HistoneH3 Recombinant Antibody (clone 1G2E4) (CAT#: ZG-0277U)

Figure 1 Mouse Anti-HistoneH3 Antibody (ZG-0277U) in WB

Positive WB detected in: Raw264.7 whole cell lysate, Raji whole cell lysate, MCF-7 whole cell lysate All lanes: HistoneH3 antibody at 1: 20000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 1min

Figure 2 Mouse Anti-HistoneH3 Antibody (ZG-0277U) in IHC

IHC image of ZG-0277U diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-HistoneH3 Antibody (ZG-0277U) in IP

Immunoprecipitating HistoneH3 in Hela whole cell lysate Lane 1: Mouse control IgG2b in Hela whole cell lysate.
Lane 2: ZG-0277U (2μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
For western blotting, the blot was detected with ZG-0277U at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000