Rabbit Anti-HP Recombinant Antibody (clone 5A5)
CAT#: ZG-0743J
This product is a rabbit antibody that recognizes HP.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-HP Antibody (ZG-0743J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 2 Rabbit Anti-HP Antibody (ZG-0743J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47750/1309_F11_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47750/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66795/170921_A_6_6_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47750/788_B12_1_red_green.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47750/118508_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (Hep G2, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000257017-HP
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Specifications
- Immunogen
- A synthesized peptide derived from human Haptoglobin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HP
- Species Reactivity
- Human
- Clone
- 5A5
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Infections
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2301MZ | Recombinant Mouse Anti-Human HP Antibody (clone 2) | ELISA, IHC-P, WB | Mouse antibody |
| HPAB-0239-YC | Mouse Anti-HP Recombinant Antibody (HPAB-0239-YC) | ELISA | Mouse IgG |
| MRO-0712-CN | Mouse Anti-HP Recombinant Antibody (clone A10-F5) | WB, IHC, ELISA | Mouse IgG1 |
| MRO-0713-CN | Mouse Anti-HP Recombinant Antibody (clone 5-D3) | WB, IHC, IF | Mouse IgG1 |
| MRO-0714-CN | Rabbit Anti-HP Recombinant Antibody (clone JM10-79) | WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0885MZ | Chicken Anti-Haptoglobin Polyclonal IgY | ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1665 | Rabbit Anti-HP Recombinant Antibody (clone DS1665AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0239-YC-S(P) | Mouse Anti-HP Recombinant Antibody; scFv Fragment (HPAB-0239-YC-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0239-YC-F(E) | Mouse Anti-HP Recombinant Antibody; Fab Fragment (HPAB-0239-YC-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1053 | Anti-HP Immunohistochemistry Kit | IHC | |
| VS-0525-XY3271 | Anti-Mouse HP Immunohistochemistry Kit | IHC | |
| VS-0525-XY3270 | Anti-Human HP Immunohistochemistry Kit | IHC |
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