Rabbit Anti-HPGD Recombinant Antibody (clone R04-5I2)
CAT#: VS3-XY3385
The antibody is a recombinant rabbit antibody binding with HPGD (15-hydroxyprostaglandin dehydrogenase), which catalyzes the NAD-dependent dehydrogenation of a broad array of hydroxylated polyunsaturated fatty acids. This product recognizes and binds to human HPGD. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/10_A5_1_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_4_6.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_5.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_5_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, ASC diff, HEL, OE19, RT4, SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164120-HPGD
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HPGD
- Species Reactivity
- Human
- Clone
- R04-5I2
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Hypertrophic Osteoarthropathy, Primary, Autosomal Recessive, 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
- Gene ID
- 3248
- UniProt ID
- P15428
- Long Name
- 15-hydroxyprostaglandin dehydrogenase
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P15428)
Modification sites at neXtProt (NX_P15428)
- Protein Refseq
- NP_000851.2
- Function
- Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed:8086429, 10837478, 16828555, 16757471, 21916491, 25586183).
Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed:25586183, 15574495).
Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation.
Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HPGD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1669 | Rabbit Anti-HPGD Recombinant Antibody (clone DS1669AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-279YJL | Rabbit Anti-HPGD Recombinant Antibody (clone CBH264) | WB, IP | Rabbit IgG |
| VS13-YC548 | Rabbit Anti-HPGD Recombinant Antibody (VS13-YC548) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1201 | AbPlus™ Anti-Hpgd Magnetic Beads (VS-0724-YC1201) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1055 | Anti-HPGD Immunohistochemistry Kit | IHC | |
| VS-0525-XY3273 | Anti-Mouse HPGD Immunohistochemistry Kit | IHC | |
| VS-0525-XY3272 | Anti-Human HPGD Immunohistochemistry Kit | IHC |
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