Rabbit Anti-HSF1 Recombinant Antibody (clone 2A7) (CAT#: ZG-0744J)
This product is a rabbit antibody that recognizes HSF1.
Specific Inquiry
WB
Figure 1 Rabbit Anti-HSF1 Antibody (ZG-0744J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate
All lanes Phospho-HSF1 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 82 KDa
Observed band size: 82 KDa
ICC
Figure 2 Rabbit Anti-HSF1 Antibody (ZG-0744J) in ICC
Immunocytochemistry analysis of this product diluted at 1:80 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-HSF1 (S326)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSF1
- Species Reactivity
- Human
- Clone
- 2A7
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HSF1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1685 | Rabbit Anti-HSF1 Recombinant Antibody (clone DS1685AB) | ICC, IHC-P, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX543 | Mouse Anti-HSF1 Recombinant Antibody (clone 4D-5F) | WB, ICC, IHC | Mouse IgG2b |
| VS3-XY825 | Mouse Anti-HSF1 Recombinant Antibody (clone 4D5F4) | ELISA, WB, IHC, ICC | Mouse IgG2b |
| VS3-XY826 | Mouse Anti-HSF1 Recombinant Antibody (clone 2D9G5) | ELISA, WB, IHC, ICC | Mouse IgG2b |
| VS3-WK836 | Rabbit Anti-HSF1 Recombinant Antibody (VS3-WK836) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS3-FY699 | Recombinant Rabbit Anti-HSF1 Antibody (clone R07-0G0) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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