Rabbit Anti-HSF1 Recombinant Antibody (clone 2A7) (CAT#: ZG-0744J)

Name: Rabbit Anti-HSF1 Recombinant Antibody (clone 2A7)
SKU: ZG-0744J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes HSF1.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-HSF1 Antibody (ZG-0744J) in WB

Figure 1 Rabbit Anti-HSF1 Antibody (ZG-0744J) in WB

Western Blot
Positive WB detected in Hela whole cell lysate
All lanes Phospho-HSF1 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 82 KDa
Observed band size: 82 KDa

ICC

Figure 2 Rabbit Anti-HSF1 Antibody (ZG-0744J) in ICC

Figure 2 Rabbit Anti-HSF1 Antibody (ZG-0744J) in ICC

Immunocytochemistry analysis of this product diluted at 1:80 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8888/41_C11_2_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_B_8_5.jpg

Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_3.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_7_5.jpg

Figure 5 Urinary bladder
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_4_5.jpg

Figure 6 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_6_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185122-HSF1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human Phospho-HSF1 (S326)

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human HSF1

Species Reactivity
Human

Clone
2A7

Applications
ELISA, WB, IHC

Conjugate
Uconjugated

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200

Target

Alternative Names
HSTF1; heat shock factor protein 1

Gene ID
3297

UniProt ID
Q00613

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibodies Rabbit Monoclonal Antibodies NHP Antibodies IHC Kits & Antibodies

CAT	Product Name	Application	Type
MOB-1134MZ	Recombinant Mouse Anti-Human HSF1 Antibody (clone K8G0)	ELISA	Mouse antibody
MRO-2336-CN	Rabbit Anti-HSF1 Recombinant Antibody (clone CBACN-627)	WB, IF, IHC, IP, FC	Rabbit IgG
VS3-XY825	Mouse Anti-HSF1 Recombinant Antibody (clone 4D5F4)	ELISA, WB, IHC, ICC	Mouse IgG2b
VS3-FY699	Recombinant Rabbit Anti-HSF1 Antibody (clone R07-0G0)	WB, IHC-P, IP	Rabbit IgG
VS3-FY700	Recombinant Rabbit Anti-HSF1 (phospho Ser326) Antibody (clone R08-4I1)	WB, IHC-Fr, IHC-P, ICC, IF, IP	Rabbit IgG

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CAT	Product Name	Application
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