Rabbit Anti-HSF1 Recombinant Antibody (clone 2A7) (CAT#: ZG-0744J)
This product is a rabbit antibody that recognizes HSF1.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-HSF1 Antibody (ZG-0744J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate
All lanes Phospho-HSF1 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 82 KDa
Observed band size: 82 KDa
ICC
Figure 2 Rabbit Anti-HSF1 Antibody (ZG-0744J) in ICC
Immunocytochemistry analysis of this product diluted at 1:80 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-HSF1 (S326)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSF1
- Species Reactivity
- Human
- Clone
- 2A7
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "HSF1"
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1685 | Rabbit Anti-HSF1 Recombinant Antibody (clone DS1685AB) | ICC, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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