Rabbit Anti-HSP90AA1 Recombinant Antibody (clone 4B5)
CAT#: ZG-0745J
This product is a rabbit antibody that recognizes HSP90AA1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-HSP90AA1 Antibody (ZG-0745J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
All lanes: Hsp90 alpha antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 99 KDa
Observed band size: 85 KDa
IHC
Figure 2 Rabbit Anti-HSP90AA1 Antibody (ZG-0745J) in IHC
IHC image of this product diluted at 1:80 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 3 Rabbit Anti-HSP90AA1 Antibody (ZG-0745J) in IHC
IHC image of this product diluted at 1:80 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 4 Rabbit Anti-HSP90AA1 Antibody (ZG-0745J) in IF
Immunofluorescence staining of NIH/3T3 cells with this product at 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 5 Rabbit Anti-HSP90AA1 Antibody (ZG-0745J) in IP
Immunoprecipitating Hsp90 in Hela whole cell lysate
Lane 1: Rabbit control IgG in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0745J (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2058/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2058/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2058/673_C12_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2058/667_C12_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2058/5779_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080824-HSP90AA1
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Specifications
- Immunogen
- A synthesized peptide derived from human HSP90AA1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSP90AA1
- Species Reactivity
- Human, Rat
- Clone
- 4B5
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p; heat shock protein HSP 90-alpha; HSP 86; LPS-associated protein 2; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; lipopolysaccharide-associated protein 2; renal carcinoma antigen NY-REN-38
- Gene ID
- 3320
- UniProt ID
- P07900
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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