Rabbit Anti-HSP90AA1 Recombinant Antibody (clone 4D1) (CAT#: ZG-0747J)
This product is a rabbit antibody that recognizes HSP90AA1.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-HSP90AA1 Antibody (ZG-0747J) in WB
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: Hsp90 alpha antibody at 1.9 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 99 KDa
Observed band size: 90 KDa
IHC
Figure 2 Rabbit Anti-HSP90AA1 Antibody (ZG-0747J) in IHC
IHC image of this product diluted at 1:190 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-HSP90AA1 Antibody (ZG-0747J) in IF
Immunofluorescence staining of NIH/3T3 cells with this product at 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human HSP90AA1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSP90AA1
- Species Reactivity
- Human
- Clone
- 4D1
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p; heat shock protein HSP 90-alpha; HSP 86; LPS-associated protein 2; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; lipopolysaccharide-associated protein 2; renal carcinoma antigen NY-REN-38
- Gene ID
- 3320
- UniProt ID
- P07900
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| MOB-0881CT | Recombinant Mouse anti-Human HSP90AA1 Monoclonal antibody (5G20) | ELISA, IHC-P, WB | |
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