Mouse Anti-HSPA1A Recombinant Antibody (VS7-0425-WR90)
CAT#: VS7-0425-WR90
This product is a mouse IgG recombinant antibody that binds to human HSPA1A. The antibody recognizes human HSP70 (N-terminus). It can be used for WB assay and cross reacts with mouse, rat, bovine, dog, goat, and pig.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in the basal layer of glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_1_5_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204389-HSPA1A
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Specifications
- Immunogen
- Recombinant human HSPA1A (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA1A
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog, Goat, Pig
- Molecule Class
- Heat Shock Protein
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Inflammatory Diseases; Neurodegenerative Diseases; Ischemia-Reperfusion Injury
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Target
- Alternative Names
- HSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
- Gene ID
- 3303
- Long Name
- Heat Shock Protein Family A (Hsp70) Member 1A
- Cellular Localization
- Cytoplasm; Cytoskeleton; Nucleus; Secreted
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_005337.2
- Function
- Chaperone; Host cell receptor for virus entry; Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HSPA1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-517 | Recombinant Anti-human HSPA1A Antibody | WB, IP, FuncS | IgG |
| ZG-0453J | Mouse Anti-HSPA1A Recombinant Antibody (ZG-0453J) | WB | Mouse IgG |
| ZG-0454J | Mouse Anti-HSPA1A Recombinant Antibody (ZG-0454J) | WB | Mouse IgG |
| ZG-0456J | Mouse Anti-HSPA1A Recombinant Antibody (ZG-0456J) | WB | Mouse IgG |
| ZG-0457J | Mouse Anti-HSPA1A Recombinant Antibody (ZG-0457J) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-517-F(E) | Recombinant Anti-human HSPA1A Antibody Fab Fragment | WB, RIA, FuncS | Fab |
| MHH-517-F(E) | Recombinant Human Anti-human HSPA1A Antibody Fab Fragment | WB, FC, FuncS | Fab |
| HPAB-0243-YC-F(E) | Mouse Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0243-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0244-YC-F(E) | Human Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0244-YC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-517-S(P) | Recombinant Anti-human HSPA1A Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-517-S(P) | Recombinant Human Anti-human HSPA1A Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| HPAB-0243-YC-S(P) | Mouse Anti-HSPA1A Recombinant Antibody scFv Fragment (HPAB-0243-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0244-YC-S(P) | Human Anti-HSPA1A Recombinant Antibody scFv Fragment (HPAB-0244-YC-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-916CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 2A4) | Block, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-917CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 3A3) | Block, ELISA, FC, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG1 |
| NEUT-918CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 4G4) | Inhib, IHC-P, ICC, IF, FC, WB | Mouse IgG1 |
| NEUT-919CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 5A5) | Block, FC, GSA, ICC, IF, IHC, IHC-Fr, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1692 | Rabbit Anti-HSPA1A Recombinant Antibody (clone DS1692AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY132 | AbPlus™ Anti-HSPA1A Magnetic Beads (2A4) | IP, Protein Purification | |
| VS-0724-YC1220 | AbPlus™ Anti-HSPA1A Magnetic Beads (VS-0724-YC1220) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY198 | Mouse Anti-HSPA1A scFv-Fc Chimera (VS-0325-FY198) | ELISA, FC | Mouse IgG1, scFv-Fc |
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