Rabbit Anti-HTRA1 Recombinant Antibody (clone R22-3C-3)
CAT#: VS3-FY2191
This recombinant monoclonal antibody demonstrates a strong affinity for human HTRA1. The antibody detected the HTRA1 protein with a calculated molecular weight of 51 kDa and an observed molecular weight of 51 kDa, it is suitable for western blot at a dilution of 1/500-1/1000 and for immunofluorescence at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/1781_F1_29_cr596dc90203c13_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/421_D4_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/427_D4_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_7_5.jpg
Normal Tissue
Figure 7 Ovary
Follicle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_5_7.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC, LHCN-M2, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166033-HTRA1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HTRA1
- Species Reactivity
- Human
- Clone
- R22-3C-3
- Applications
- Western Blot, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 51 kDa, while the observed molecular weight stands at 51kDa.
- Related Disease
- Cerebral Arteriopathy, Autosomal Recessive, With Subcortical Infarcts And Leukoencephalopathy and Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy, Type 2
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- HtrA Serine Peptidase 1; High-Temperature Requirement A Serine Peptidase 1; Protease, Serine, 11 (IGF Binding); PRSS11; HtrA; L56; Serine Protease HTRA1; Serine Protease 11; IGFBP5-Protease
- Gene ID
- 5654
- UniProt ID
- Q92743
- Sequence Similarities
- Belongs to the peptidase S1C family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Secreted
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q92743)
Modification sites at neXtProt (NX_Q92743)
- Protein Refseq
- NP_002766.1
- Function
- Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1362-sdAb | Recombinant Anti-Human HTRA1 VHH Single Domain Antibody | IP, ChiP, IHC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-113CT | Human Anti-HtrA1 Recombinant Antibody (TAB-113CT) | ELISA, WB | Human IgG1 |
| TAB-113CT-S(P) | Human Anti-HtrA1 Recombinant Antibody; scFv Fragment (TAB-113CT-S(P)) | ELISA, WB | Human scFv |
| TAB-113CT-F(E) | Human Anti-HtrA1 Recombinant Antibody; Fab Fragment (TAB-113CT-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0526-CN | Mouse Anti-HTRA1 Recombinant Antibody (HPAB-0526-CN) | FuncS, WB, Inhib | Mouse IgG2a |
| HPAB-0527-CN | Mouse Anti-HTRA1 Recombinant Antibody (HPAB-0527-CN) | IHC, WB, Inhib | Mouse IgG2a |
| HPAB-0529-CN | Human Anti-HTRA1 Recombinant Antibody (HPAB-0529-CN) | WB, ELISA, FC, IHC | Humanized IgG |
| MOB-092CQ | Mouse Anti-HTRA1 Recombinant Antibody (clone YC109) | ELISA, WB, IF, IP | Mouse IgG1, κ |
| VS-0723-WK18 | Human Anti-HTRA1 Recombinant Antibody (clone Galegenimab) | FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3081LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3081LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3082LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3082LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3083LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3083LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3084LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3084LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3085LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3085LY-S(P)) | ELISA, Block | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3081LY-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-3081LY-F(E)) | ELISA, Block | Mouse Fab |
| HPAB-3082LY-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-3082LY-F(E)) | ELISA, Block | Mouse Fab |
| HPAB-3083LY-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-3083LY-F(E)) | ELISA, Block | Mouse Fab |
| HPAB-3084LY-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-3084LY-F(E)) | ELISA, Block | Mouse Fab |
| HPAB-3085LY-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-3085LY-F(E)) | ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC147 | Recombinant Anti-HTRA1 Eliminating Antibody, pH-Sensitive (VS-0625-YC147) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
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