Rabbit Anti-IDE Recombinant Antibody (clone R04-4E8)
CAT#: VS3-FY2017
This particular antibody is a rabbit recombinant monoclonal variant with a strong binding affinity to human IDE. It is recommended for utilization in a range of assays, such as WB and ICC/IF. The antibody targeting insulin degrading enzyme was identified through immunocytochemistry analysis of Insulin degrading enzyme/IDE (green) in hela cells.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human stomach
Immunohistochemical staining of human stomach shows membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_1_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membrano
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_6_6.jpg
Normal Tissue
Figure 7 Skin
Melanocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membrano
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_B_7_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63478/143063_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119912-IDE
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ICC
Figure 1 Anti-IDE Antibody (VS3-FY2017) in ICC.
Immunocytochemistry staining of Insulin degrading enzyme/IDE (green) in hela using the anti-Insulin degrading enzyme/IDE antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human insulin degrading enzyme/IDE
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IDE
- Species Reactivity
- Human
- Clone
- R04-4E8
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 118 kDa, while the observed molecular weight stands at 118 kDa.
- Related Disease
- Alzheimer's diseases
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Insulin Degrading Enzyme
- Gene ID
- 3416
- UniProt ID
- P14735
- Sequence Similarities
- Belongs to the peptidase M16 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Secreted
- Post Translation Modifications
- The N-terminus is blocked. (P14735-IDE_HUMAN)
Ubiquitination at Lys192, Lys308, Lys884, Lys929, Lys938, and Lys1018 (NX_P14735 [NX_P14735-1])
Modification sites at PhosphoSitePlus (P14735)
Glycosylation from GlyGen (P14735) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016871676.1; XP_016871677.1; XP_016871679.2; XP_047281125.1; XP_047281127.1
- Function
- Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia. Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IDE"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-535 | Recombinant Anti-human IDE Antibody | WB, Dot, FuncS | IgG |
| PABL-594 | Mouse Anti-IDE Recombinant Antibody (clone YC112) | WB, FuncS | Mouse IgG |
| MRO-0844-CN | Rabbit Anti-IDE Recombinant Antibody (clone JJ0949) | WB, IHC | Rabbit IgG |
| PABS-0055 | Mouse Anti-IDE Recombinant Antibody (Clone Fab(IDE)) | ELISA, FuncS | Mouse IgG |
| VS4-WK236 | Mouse Anti-IDE Recombinant Antibody (VS4-WK236) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-535-F(E) | Recombinant Anti-human IDE Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| MHH-535-F(E) | Recombinant Human Anti-human IDE Antibody Fab Fragment | ELISA, RIA, FuncS | Fab |
| PFBL-589 | Mouse Anti-IDE Recombinant Antibody (clone YC113); Fab Fragment | WB, FuncS | Mouse Fab |
| PFBS-0055 | Mouse Anti-IDE Recombinant Antibody (Clone Fab(IDE)); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-535-S(P) | Recombinant Anti-human IDE Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-535-S(P) | Recombinant Human Anti-human IDE Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| PSBL-589 | Mouse Anti-IDE Recombinant Antibody (clone YC113); scFv Fragment | WB, FuncS | Mouse scFv |
| PSBS-0055 | Mouse Anti-IDE Recombinant Antibody (Clone Fab(IDE)); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-110 | Recombinant Human Anti-IDE Antibody | WB, Neut, FuncS | IgG |
| PABX-110-S (P) | Recombinant Human Anti-IDE Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PABX-110-F (E) | Recombinant Human Anti-IDE Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1732 | Rabbit Anti-IDE Recombinant Antibody (clone DS1732AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY267 | Mouse Anti-NHP IDE Recombinant Antibody (clone OTI4E5) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3380 | Anti-IDE Immunohistochemistry Kit | IHC | |
| VS-0525-XY3381 | Anti-Mouse IDE Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC149 | Recombinant Anti-IDE Eliminating Antibody, pH-Sensitive (VS-0625-YC149) | Antigen-Sweeping In Vivo. | Human IgG |
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