Rabbit Anti-IGFBP2 Recombinant Antibody (clone JU37-46)
CAT#: MRO-0819-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to IGFBP2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77723/1615_H9_6_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (ASC diff, HMC-1, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115457-IGFBP2
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Specifications
- Immunogen
- Recombinant protein within mouse IGFBP2, aa 1-250
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IGFBP2
- Species Reactivity
- Human
- Clone
- JU37-46
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Excellent IHC Staining
We applied this Rabbit Anti-IGFBP2 antibody (clone JU37-46) for immunohistochemistry on human tissue samples. The staining was specific and distinct, allowing us to visualize the localization of IGFBP2 effectively. The recombinant nature of this monoclonal antibody gave us confidence in the specificity, eliminating the variability we often see with traditional polyclonal options.
Clear Bands In Western Blot
Our lab used this antibody for Western Blot analysis to detect IGFBP2 expression in various cell lysates. We observed a clean band at the expected molecular weight, confirming the antibody's sensitivity. The provided buffer formulation with BSA and Glycerol seems to preserve the antibody activity well during storage at -20°C.
Q&As
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What is the host species and isotype of the clone JU37-46?
A: The clone JU37-46 is a rabbit monoclonal antibody. It possesses the Rabbit IgG isotype and is produced as a recombinant antibody. It is purified using Protein A affinity chromatography to ensure high quality and purity for your experiments.
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Can this antibody be used for Western Blotting and what is the target specificity?
A: Yes, this antibody is validated for use in Western Blot (WB) and Immunohistochemistry (IHC). It is capable of specifically binding to Human IGFBP2 (Insulin Like Growth Factor Binding Protein 2). It was raised against a recombinant protein fragment of IGFBP2.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0401-YC-S(P) | Mouse Anti-IGFBP2 Recombinant Antibody; scFv Fragment (HPAB-0401-YC-S(P)) | ELISA, Neut, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0401-YC-F(E) | Mouse Anti-IGFBP2 Recombinant Antibody; Fab Fragment (HPAB-0401-YC-F(E)) | ELISA, Neut, FuncS | Mouse Fab |
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