Recombinant Mouse Anti-IgM Antibody (1.14 ) (CAT#: FAMAB-0036-CN)

Figure 1 CD79 accessory molecules are required for rIgd surface expression on catfish 3B11 B cells.

Transfected catfish 3B11 B cells were lysed with 2% octyl-b-glucoside and immunoprecipitated using Dyna beads coated with anti-HA, anti-FLAG, or anti-trout IgM 1.14 (negative control) mAbs. Arrows denote identified proteins. IP, mAbs used for immunoselection; WB, mAbs used in Western blotting.

Edholm, E. S., Bengtén, E., Stafford, J. L., Sahoo, M., Taylor, E. B., Miller, N. W., & Wilson, M. (2010). Identification of two IgD+ B cell populations in channel catfish, Ictalurus punctatus. The Journal of Immunology, 185(7), 4082-4094.

Figure 2 IgM and IgL expression in catfish PBL.

Freshly isolated PBL and PBL depleted of surface F and G positive cells (F−/G−) by MACS were analyzed by flow cytometry. Aliquots from the unsorted (top panel) and sorted (bottom panel) populations were stained with anti-catfish IgM 9E1, anti-catfish F, and anti-catfish G mAbs. Antirainbow trout IgM 1.14 was the isotype control. The total and negatively sorted PBL populations were from the same fish.

Edholm, E. S., Wilson, M., Sahoo, M., Miller, N. W., Pilström, L., Wermenstam, N. E., & Bengtén, E. (2009). Identification of Igσ and Igλ in channel catfish, Ictalurus punctatus, and Igλ in Atlantic cod, Gadus morhua. Immunogenetics, 61(5), 353-370.