Rabbit Anti-IKBKB Recombinant Antibody (clone 3E1)
CAT#: ZG-0764J
This product is a rabbit antibody that recognizes IKBKB.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-IKBKB Antibody (ZG-0764J) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate
All lanes: IKK beta antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
IHC
Figure 2 Rabbit Anti-IKBKB Antibody (ZG-0764J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-IKBKB Antibody (ZG-0764J) in IF
Immunofluorescence staining of Hela Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1249/if_selected.jpg
Normal Tissue
Figure 2 Cerebellum.
High expression in cerebellum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_B_8_8_val_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_7_8.jpg
Normal Tissue
Figure 5 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_3_8.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104365-IKBKB
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Specifications
- Immunogen
- A synthesized peptide derived from human IKK beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IKBKB
- Species Reactivity
- Human
- Clone
- 3E1
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- IKK2; IKKB; IMD15; IMD15A; IMD15B; NFKBIKB; IKK-beta; inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta; serine/threonine protein kinase IKBKB
- Gene ID
- 3551
- UniProt ID
- O14920
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "IKBKB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0884CT | Recombinant Mouse anti-Human IKBKB Monoclonal antibody (20B0C7) | IP, WB | |
| VS3-XY851 | Mouse Anti-IKBKB Recombinant Antibody (clone 10A2) | ELISA, WB | Mouse IgG1 |
| VS3-XY852 | Mouse Anti-IKBKB Recombinant Antibody (clone 10A2C5B3) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY744 | Recombinant Rabbit Anti-IKBKB Antibody (clone R01-9H6) | WB, ICC, IF | Rabbit IgG |
| VS3-FY745 | Recombinant Rabbit Anti-IKBKB Antibody (clone R04-2E8) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0274MZ | Chicken Anti-IKBKB Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1781 | Rabbit Anti-IKBKB Recombinant Antibody (clone DS1781AB) | WB, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY146 | AbPlus™ Anti-IKBKB Magnetic Beads (VS-0424-XY146) | IP, Protein Purification | |
| VS-0724-YC981 | AbPlus™ Anti-IKBKB Magnetic Beads (VS-0724-YC981) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY275 | Rabbit Anti-NHP IKBKB Recombinant Antibody (VS-1024-XY275) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1113 | Anti-IKBKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY3470 | Anti-Mouse IKBKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY3469 | Anti-Human IKBKB Immunohistochemistry Kit | IHC |
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