Rabbit Anti-IL1RN Recombinant Antibody (clone R01-6J5)
CAT#: VS3-FY1749
Clone R01-6J5 is a rabbit recombinant monoclonal antibody that detects human IL1RN. It cross-reacts with mouse. The antibody is recommended for detection of IL1RN protein by WB, IHC-F, IHC-P, ICC/IF, IP. The recommended dilution ratio is as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This IL-1 receptor antagonist protein antibody has been teted positive by western blot analysis of IL-1RA in K562, 3T3, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1482/1422_G7_6_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1482/4971_B_5_1.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1482/4626_A_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (A-431, HaCaT, RT4, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136689-IL1RN
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WB
Figure 1 Anti-IL1RN Antibody (VS3-FY1749) in WB.
The IL-1RA expression in K562, 3T3, Hela lysates were detected using the anti-IL-1RA by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human IL1RA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse IL1RN
- Species Reactivity
- Human, Mouse
- Clone
- R01-6J5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 20 kDa, while the observed molecular weight stands at 20 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Interleukin 1 Receptor Antagonist
- Gene ID
- 3557
- UniProt ID
- P18510
- Sequence Similarities
- Belongs to the IL-1 family.
- Cellular Localization
- Cytoplasm, Secreted
- Post Translation Modifications
- Disulfide bond
Glycoprotein
- Protein Refseq
- XP_011509423.1; XP_047300140.1; XP_047300141.1; XP_047300142.1; NP_000568.1
- Function
- Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IL1RN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1402z | Mouse Anti-IL1RN Recombinant Antibody (clone 8C2) | WB, IHC | Mouse IgG2b |
| MOB-0238CT | Recombinant Mouse anti-Human IL1RN Monoclonal antibody (B82C7E22) | ELISA | |
| VS3-WK1726 | Recombinant Rabbit Anti-IL1RN Capture Antibody (clone R02-6W5) | ELISA | Rabbit IgG |
| VS3-WK1727 | Recombinant Rabbit Anti-IL1RN Detection Antibody (clone R05-7M2) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1305CQ | Mouse Anti-IL1RN Recombinant Antibody (clone CBL417) | ELISA, Neut | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1800 | Rabbit Anti-IL1RN Recombinant Antibody (clone DS1800AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3509 | Anti-IL1RN Immunohistochemistry Kit | IHC | |
| VS-0525-XY3510 | Anti-Mouse IL1RN Immunohistochemistry Kit | IHC |
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