Rabbit Anti-ILF3 Recombinant Antibody (clone 7H8)
CAT#: ZG-0058U
The purified Rabbit Anti-ILF3 Recombinant Antibody (clone 7H8) displayed high specificity against the ILF3 protein of human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-ILF3 Antibody (ZG-0058U) in IHC
IHC image of ZG-0058U diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-ILF3 Antibody (ZG-0058U) in IF
Immunofluorescence staining of Hela Cells with ZG-0058U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_4_6.jpg
Normal Tissue
Figure 7 Prostate
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_2_5.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1897/6416_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129351-ILF3
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Specifications
- Immunogen
- A synthesized peptide derived from human ILF3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ILF3
- Species Reactivity
- Human
- Clone
- 7H8
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Childhood Endodermal Sinus Tumor
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80; ILF3; DRBF MPHOSPH4 NF90
- Gene ID
- 3609
- UniProt ID
- Q12906
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ILF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1820 | Rabbit Anti-ILF3 Recombinant Antibody (clone DS1820AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0837-CN | Rabbit Anti-ILF3 Recombinant Antibody (clone JG37-63) | WB, IP, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1122 | Anti-ILF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3547 | Anti-Human ILF3 Immunohistochemistry Kit | IHC |
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