Rabbit Anti-IMPDH2 Recombinant Antibody (clone R06-1B8)
CAT#: VS3-FY2015
The R06-1B8 recombinant monoclonal antibody specifically attaches to human IMPDH2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We validate the QC result of the antibody using western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This IMPDH2 antibody has been teted positive by western blot analysis of IMPDH2 in 3T3, mouse liver lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20717/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20717/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1400/5501_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1400/5501_B_9_5.jpg
Normal Tissue
Figure 5 Small intestine
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1400/5501_A_4_2.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1400/5501_A_9_3.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20717/46628_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000178035-IMPDH2
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WB
Figure 1 Anti-IMPDH2 Antibody (VS3-FY2015) in WB.
The IMPDH2 expression in 3T3, mouse liver lysates were detected using the anti-IMPDH2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human IMPDH2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat IMPDH2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-1B8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 56 kDa, while the observed molecular weight stands at 56 kDa.
- Related Disease
- Impdh2 Enzyme Activity, Variation In and Gtp Cyclohydrolase 1-Deficient Dopa-Responsive Dystonia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Inosine Monophosphate Dehydrogenase 2
- Gene ID
- 3615
- UniProt ID
- P12268
- Sequence Similarities
- Belongs to the IMPDH/GMPR family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated leading to its degradation by the proteasome. (P12268-IMDH2_HUMAN)
The N-terminus is blocked. (P12268-IMDH2_HUMAN)
Acetylated by CLOCK in a circadian manner (PubMed:28985504). (P12268-IMDH2_HUMAN)
Ubiquitination at Lys109, Lys124, Lys134, Lys195, Lys208, Lys293, Lys311, Lys349, Lys422, Lys450, Lys455, Lys474, and Lys489 (NX_P12268 [NX_P12268-1])
Modification sites at PhosphoSitePlus (P12268)
Glycosylation from GlyGen (P12268) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000875.2; NP_001397688.1; NP_001397689.1; NP_001397690.1
- Function
- Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MRO-0840-CN | Rabbit Anti-IMPDH2 Recombinant Antibody (clone JE40-87) | WB, IF, IHC, FC, IP | Rabbit IgG |
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