Rabbit Anti-IRF2 Recombinant Antibody (VS3-CJ653)
CAT#: VS3-CJ653
This product is a rabbit antibody that recognizes human, and mouse IRF2.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30813/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57327/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/924_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30813/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_3_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66140_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_5.jpg
Normal Tissue
Figure 9 Urinary bladder
Urothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_4_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168310-IRF2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse IRF2
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence.
Target
- Alternative Names
- IRF-2
- Gene ID
- 3660
- UniProt ID
- P14316
- Sequence Similarities
- Belongs to the IRF family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
- Protein Refseq
- NP_002190.2
- Function
- Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-355 | Recombinant Human Anti-Human IRF2 Antibody, clone T307 | IF, ELISA | IgG |
| MOB-0995CT | Recombinant Mouse anti-Human IRF2 Monoclonal antibody (EML466) | ICC, IF, IHC-P, WB | |
| MRO-0868-CN | Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (CBACN-320) | WB, IF | Rabbit IgG |
| VS-0923-FY197 | Recombinant Mouse Anti-IRF2 Antibody (VS-0923-FY197) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1854 | Hi-Affi™ Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (DS1854AB) | WB, IHC | IgG |
| MOR-4633 | Hi-Affi™ Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (TH146DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1142 | Anti-IRF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3585 | Anti-Human IRF2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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