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  • Rabbit Anti-IRF2 Recombinant Antibody (VS3-CJ653)

Rabbit Anti-IRF2 Recombinant Antibody (VS3-CJ653) (CAT#: VS3-CJ653)

This product is a rabbit antibody that recognizes human, and mouse IRF2.


See more Engineered Antibody products for IRF2
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  • Gene Expression
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30813/if_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57327/if_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/924_G8_1_selected.jpg
Figure 4 IHC staining of human thyroid gland
Figure 4 IHC staining of human thyroid gland
(Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30813/ihc_selected.jpg
Figure 5 IHC staining of human colon
Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_3_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66140_B_7_5.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_3.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_7_5.jpg
Figure 9 Urinary bladder
Figure 9 Urinary bladder
(Urothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_4_5.jpg
Figure 10 Testis
Figure 10 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_4_6.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32306/66200_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Figure 12 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168310-IRF2

Specifications

  • Immunogen
  • Recombinant protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse IRF2
  • Species Reactivity
  • Human, Mouse
  • Applications
  • WB, ICC, IF
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 40% Glycerol, 1% BSA, TBS, pH7.4.
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence.

Target

  • Alternative Names
  • IRF-2
  • Sequence Similarities
  • Belongs to the IRF family.
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
    The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
  • Function
  • Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IRF2"

Recombinant Antibody

CAT Product Name Application Type
DrMAB-355 Recombinant Human Anti-Human IRF2 Antibody, clone T307 IF, ELISA IgG
MOB-0995CT Recombinant Mouse anti-Human IRF2 Monoclonal antibody (EML466) ICC/IF, IHC-P, WB
MRO-0868-CN Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (CBACN-320) WB, IF Rabbit IgG
VS-0923-FY197 Recombinant Mouse Anti-IRF2 Antibody (VS-0923-FY197) ELISA, WB Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1854 Hi-Affi™ Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (DS1854AB) WB, IHC IgG
MOR-4633 Hi-Affi™ Recombinant Rabbit Anti-IRF2 Monoclonal Antibody (TH146DS) WB, IF, ICC, IHC-P, FC, ChIP IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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