Rabbit Anti-ITK Recombinant Antibody (clone R04-8H3) (CAT#: VS3-XY3402)
It is a recombinant rabbit antibody specific for ITK (IL2 inducible T cell kinase), which regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. It specifically targets human ITK. This product has been tested using Western Blot, Immunohistochemistry-Paraffin and Flow cytometry. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Flow cytometry is 1/500-1/1000, 1/50-1/100 and 1/50-1/100, respectively.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ITK
- Species Reactivity
- Human
- Clone
- R04-8H3
- Applications
- WB, IHC-P, FC
- Related Disease
- Lymphoproliferative Syndrome 1; Lymphoproliferative Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- EMT; LYK; LPFS1; PSCTK2
- Gene ID
- 3702
- UniProt ID
- Q08881
- Long Name
- IL2 inducible T cell kinase
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK.
Subsequent autophosphorylation at Tyr-180 leads to the kinase activation.
The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
(Q08881-ITK_HUMAN)
Ubiquitinated. (Q08881-ITK_HUMAN)
Ubiquitination at Lys119, Lys129, Lys138, Lys150, Lys242, Lys258, Lys280, Lys291, Lys310, Lys344, Lys412, Lys497, Lys519, and Lys523 (NX_Q08881 [NX_Q08881-1])
Modification sites at PhosphoSitePlus (Q08881)
- Protein Refseq
- NP_005537.3
- Function
- Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
Phosphorylation leads to ITK autophosphorylation and full activation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1890 | Hi-Affi™ Recombinant Rabbit Anti-ITK Monoclonal Antibody (DS1890AB) | WB, FC, IP | IgG |
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