Rabbit Anti-ITK Recombinant Antibody (clone R04-8H3)
CAT#: VS3-XY3402
It is a recombinant rabbit antibody specific for ITK (IL2 inducible T cell kinase), which regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. It specifically targets human ITK. This product has been tested using Western Blot, Immunohistochemistry-Paraffin and Flow cytometry. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Flow cytometry is 1/500-1/1000, 1/50-1/100 and 1/50-1/100, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ITK
- Species Reactivity
- Human
- Clone
- R04-8H3
- Applications
- WB, IHC-P, FC
- Related Disease
- Lymphoproliferative Syndrome 1; Lymphoproliferative Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- EMT; LYK; LPFS1; PSCTK2
- Gene ID
- 3702
- UniProt ID
- Q08881
- Long Name
- IL2 inducible T cell kinase
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK.
Subsequent autophosphorylation at Tyr-180 leads to the kinase activation.
The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
(Q08881-ITK_HUMAN)
Ubiquitinated. (Q08881-ITK_HUMAN)
Ubiquitination at Lys119, Lys129, Lys138, Lys150, Lys242, Lys258, Lys280, Lys291, Lys310, Lys344, Lys412, Lys497, Lys519, and Lys523 (NX_Q08881 [NX_Q08881-1])
Modification sites at PhosphoSitePlus (Q08881)
- Protein Refseq
- NP_005537.3
- Function
- Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
Phosphorylation leads to ITK autophosphorylation and full activation.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3402. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ITK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1890 | Hi-Affi™ Recombinant Rabbit Anti-ITK Monoclonal Antibody (DS1890AB) | WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0288J | Mouse Anti-ITK Recombinant Antibody (ZG-0288J) | WB, ELISA | Mouse IgG |
| ZG-0777J | Rabbit Anti-ITK Recombinant Antibody (clone 8F10) | ELISA, IHC | Rabbit IgG |
| VS3-QX612 | Mouse Anti-ITK Recombinant Antibody (clone 5G12C4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY945 | Mouse Anti-ITK Recombinant Antibody (clone 5G6) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR166 | Mouse Anti-ITK Monoclonal Antibody (VS7-0425-WR166) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3670 | Anti-ITK Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FuncS, Inhib, IP, ELISA
-3.png)
Application: ELISA, FC, Block, SPR
-4.jpg)
Application: FC, FuncS, IA, IF, IP, IHC
-2.png)
Application: ELISA
Application: ELISA, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
