Mouse Anti-ITK Monoclonal Antibody (VS7-0425-WR166)
CAT#: VS7-0425-WR166
This product is a mouse IgG antibody that binds to human ITK. The antibody can be used for WB, IF, ICC, FC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
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Specifications
- Immunogen
- Recombinant human ITK fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITK
- Species Reactivity
- Human
- Molecule Class
- Kinase
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- EMT; LYK; LPFS1; PSCTK2
- Gene ID
- 3702
- UniProt ID
- Q08881
- Long Name
- IL2 inducible T cell kinase
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK.
Subsequent autophosphorylation at Tyr-180 leads to the kinase activation.
The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
(Q08881-ITK_HUMAN)
Ubiquitinated. (Q08881-ITK_HUMAN)
Ubiquitination at Lys119, Lys129, Lys138, Lys150, Lys242, Lys258, Lys280, Lys291, Lys310, Lys344, Lys412, Lys497, Lys519, and Lys523 (NX_Q08881 [NX_Q08881-1])
Modification sites at PhosphoSitePlus (Q08881)
- Protein Refseq
- NP_005537.3
- Function
- Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
Phosphorylation leads to ITK autophosphorylation and full activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ITK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1890 | Hi-Affi™ Recombinant Rabbit Anti-ITK Monoclonal Antibody (DS1890AB) | WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0288J | Mouse Anti-ITK Recombinant Antibody (ZG-0288J) | WB, ELISA | Mouse IgG |
| ZG-0777J | Rabbit Anti-ITK Recombinant Antibody (clone 8F10) | ELISA, IHC | Rabbit IgG |
| VS3-QX612 | Mouse Anti-ITK Recombinant Antibody (clone 5G12C4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY945 | Mouse Anti-ITK Recombinant Antibody (clone 5G6) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-XY3402 | Rabbit Anti-ITK Recombinant Antibody (clone R04-8H3) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3670 | Anti-ITK Immunohistochemistry Kit | IHC |
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