Mouse Anti-ITK Monoclonal Antibody (VS7-0425-WR166) (CAT#: VS7-0425-WR166)
This product is a mouse IgG antibody that binds to human ITK. The antibody can be used for WB, IF, ICC, FC, and ELISA assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43670/1970_G1_6_selected.jpg
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113263-ITK
Specifications
- Immunogen
- Recombinant human ITK fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITK
- Species Reactivity
- Human
- Molecule Class
- Kinase
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- EMT; LYK; LPFS1; PSCTK2
- Gene ID
- 3702
- UniProt ID
- Q08881
- Long Name
- IL2 inducible T cell kinase
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK.
Subsequent autophosphorylation at Tyr-180 leads to the kinase activation.
The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
(Q08881-ITK_HUMAN)
Ubiquitinated. (Q08881-ITK_HUMAN)
Ubiquitination at Lys119, Lys129, Lys138, Lys150, Lys242, Lys258, Lys280, Lys291, Lys310, Lys344, Lys412, Lys497, Lys519, and Lys523 (NX_Q08881 [NX_Q08881-1])
Modification sites at PhosphoSitePlus (Q08881)
- Protein Refseq
- NP_005537.3
- Function
- Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
Phosphorylation leads to ITK autophosphorylation and full activation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1890 | Hi-Affi™ Recombinant Rabbit Anti-ITK Monoclonal Antibody (DS1890AB) | WB, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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