Mouse Anti-JMJD6 Recombinant Antibody (ZG-036R)
CAT#: ZG-036R
This product is a mouse antibody that recognizes JMJD6. It can be used in a wide variety of applications, such as: WB, IF.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-JMJD6 Antibody (ZG-036R) in WB
Western Blot analysis using PSR monoclonal antibody against HeLa, HL-60 cell lysate.
IF
Figure 2 Mouse Anti-JMJD6 Antibody (ZG-036R) in IF
Immunofluorescence analysis of HeLa cells using PSR monoclonal antibody.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1020_D11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1043_F5_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/993_D11_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000070495-JMJD6
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Specifications
- Immunogen
- Purified recombinant human PSR (N-terminus) protein fragments expressed in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog
- Applications
- WB, IF
Product Property
- Purification
- Affinity purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:3000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| DrMAB-362 | Recombinant Human Anti-Human JMJD6 Antibody Fab Fragment, clone D279 | IP, IF, ELISA, IP-MS | Fab |
| PFBL-618 | Mouse Anti-JMJD6 Recombinant Antibody; Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
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| PABL-623 | Mouse Anti-JMJD6 Recombinant Antibody | WB, IHC, IF, FuncS | Mouse IgG |
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| CAT | Product Name | Application | Type |
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| PSBL-618 | Mouse Anti-JMJD6 Recombinant Antibody; scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
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| PABX-127 | Recombinant Hamster Anti-JMJD6 Antibody | WB, ELISA, IF, FuncS | IgG |
| PABX-127-F (E) | Recombinant Hamster Anti-JMJD6 Antibody Fab Fragment | WB, ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-127-S (P) | Recombinant Hamster Anti-JMJD6 Antibody scFv Fragment | WB, ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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