Mouse Anti-JUP Monoclonal Antibody (clone 4C12)
CAT#: VS7-0425-WR173
This product is a mouse IgG1 antibody that binds to human JUP. The antibody 4C12 can be used for WB, IHC, and ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cell junctions & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2139/674_A12_4_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/122459_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/122459_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/122459_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/122459_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32047/122459_A_5_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2139/5787_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HaCaT, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173801-JUP
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Specifications
- Immunogen
- Recombinant human JUP fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human JUP
- Species Reactivity
- Human
- Clone
- 4C12
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated
- Related Disease
- Naxos disease (NXD); Arrhythmogenic right ventricular dysplasia, familial, 12 (ARVD12)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- JUP; Catenin Gamma; Catenin (Cadherin-Associated Protein), Gamma 80kDa; DPIII; DP3; Junction Plakoglobin; Desmoplakin-3; PKGB; ARVD12; Desmoplakin III; CTNNG
- Gene ID
- 3728
- UniProt ID
- P14923
- Long Name
- Junction plakoglobin
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Cytoskeleton; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001339702.1
- Function
- Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| VS3-QX625 | Mouse Anti-JUP Recombinant Antibody (clone 4B4-C7-E9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1170 | Anti-JUP Immunohistochemistry Kit | IHC | |
| VS-0525-XY3708 | Anti-Mouse JUP Immunohistochemistry Kit | IHC | |
| VS-0525-XY3707 | Anti-Human JUP Immunohistochemistry Kit | IHC | |
| VS-0525-XY3709 | Anti-Rat JUP Immunohistochemistry Kit | IHC |
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