Rabbit Anti-KDM4B Recombinant Antibody (clone R08-9G9)
CAT#: VS3-FY1756
This marvelous recombinant antibody is crucial for validating in immunohistochemistry (IHC) studies. As a rabbit recombinant monoclonal antibody that is designed to target human KDM4B. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This R08-9G9 antibody is validated for western blot analysis of KDM4B in 293, COS7 lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71568/1923_A10_32_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71568/2031_A2_1_red_green.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000127663-KDM4B
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WB
Figure 1 Anti-KDM4B Antibody (VS3-FY1756) in WB.
The KDM4B expression in 293, COS7 lysates were detected using the anti-KDM4B by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human KDM4B/JMJD2B
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KDM4B
- Species Reactivity
- Human
- Clone
- R08-9G9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 122 kDa, while the observed molecular weight stands at 150 kDa.
- Related Disease
- Intellectual Developmental Disorder, Autosomal Dominant 65 and Non-Specific Syndromic Intellectual Disability
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Lysine Demethylase 4B; JmjC Domain-Containing Histone Demethylation Protein 3B; Jumonji Domain-Containing Protein 2B; Lysine (K)-Specific Demethylase 4B; Jumonji Domain Containing 2B; Tudor Domain Containing 14B; JMJD2B
- Gene ID
- 23030
- UniProt ID
- O94953
- Sequence Similarities
- Belongs to the JHDM3 histone demethylase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation
Phosphoprotein
- Protein Refseq
- XP_005259578.2; XP_011526116.1; XP_011526119.1; XP_011526121.1; XP_011526122.1
- Function
- Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KDM4B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-376 | Recombinant Human Anti-Human KDM4B Antibody, clone D437 | ELISA, IP-MS | IgG |
| VS7-0425-WR202 | Mouse Anti-KDM4B Recombinant Antibody (clone 3A6H6) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC616 | CytoStream™ Rabbit Anti-KDM4B Recombinant Antibody (VS13-YC616) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3753 | Anti-KDM4B Immunohistochemistry Kit | IHC | |
| VS-0525-XY3754 | Anti-Mouse KDM4B Immunohistochemistry Kit | IHC |
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