Mouse Anti-KIF11 Recombinant Antibody (VS3-FY2750)
CAT#: VS3-FY2750
The anti-KIF11 antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the KIF11 protein, a calculated weight of 119 kDa. It exhibits cross-reactivity with human. This monoclonal antibody is potential for conducting research on Microcephaly with or without Chorioretinopathy, Lymphedema, Or Impaired Intellectual Development and Microcephaly and Chorioretinopathy, Autosomal Recessive, 1. The antibody can be utilized for detection of KIF11 by Western Blot, Immunohistochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10093/1401_H1_6_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_9_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_B_8_5.jpg
Normal Tissue
Figure 4 Oral mucosa
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_7_1.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_8_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138160-KIF11
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Kinesin family member 11 of human
- Species Reactivity
- Human
- Applications
- WB, IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 119 kDa.
- Related Disease
- Microcephaly With Or Without Chorioretinopathy, Lymphedema, Or Impaired Intellectual Development and Microcephaly And Chorioretinopathy, Autosomal Recessive, 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Kinesin Family Member 11; Thyroid Receptor-Interacting Protein 5; Kinesin-Like Spindle Protein HKSP; Kinesin-Related Motor Protein Eg5; TR-Interacting Protein 5; Kinesin-Like Protein 1; TRIP-5; KNSL1
- Gene ID
- 3832
- Sequence Similarities
- Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
- Cellular Localization
- Cytoplasm, cytoskeleton, spindle pole
- Post Translation Modifications
- Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Ubiquitinated at Lys-1034 by UHRF1 via 'Lys-63'-linked ubiquitin chains, leading to interaction with spindle assembly factor TPX2, thereby ensuring accurate distribution to the spindles during metaphase.
Ubiquitination at Lys1034
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_004514.2
- Function
- Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, 37728657).
Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KIF11"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3056z | Mouse Anti-KIF11 Recombinant Antibody (clone 23G5) | WB, ELISA, ICC | Mouse IgG1 |
| ZG-0274J | Mouse Anti-KIF11 Recombinant Antibody (ZG-0274J) | WB, IF | Mouse IgG |
| ZG-0275J | Mouse Anti-KIF11 Recombinant Antibody (ZG-0275J) | WB, ICC | Mouse IgG |
| ZG-0785J | Rabbit Anti-KIF11 Recombinant Antibody (clone 1G11) | ELISA, WB | Rabbit IgG |
| VS3-WK684 | Rabbit Anti-KIF11 Recombinant Antibody (clone JU83-16) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1934 | Hi-Affi™ Recombinant Rabbit Anti-KIF11 Monoclonal Antibody (DS1934AB) | ICC, IF, IP, WB | IgG |
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