Mouse Anti-KIF22 Monoclonal Antibody (clone 5F3) (CAT#: VS7-0425-WR214)
This product is a mouse IgG1 antibody that binds to human KIF22. The antibody 5F3 can be used for WB and IHC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48213/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48213/if_selected.jpg
Figure 2 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_8_4.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_8_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75670/169015_A_7_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079616-KIF22
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079616-KIF22
Specifications
- Immunogen
- Recombinant human KIF22 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KIF22
- Species Reactivity
- Human
- Clone
- 5F3
- Molecule Class
- Kinesin-Like Protein
- Applications
- WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Spondyloepimetaphyseal dysplasia with joint laxity, 2 (SEMDJL2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
- Gene ID
- 3835
- UniProt ID
- Q14807
- Long Name
- Kinesin Family Member 22
- Cellular Localization
- Cytoplasm; Cytoskeleton; Microtubule; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001243198.1
- Function
- DNA-binding; Motor protein
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1936 | Hi-Affi™ Recombinant Rabbit Anti-KIF22 Monoclonal Antibody (DS1936AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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