Mouse Anti-KLF1 Recombinant Antibody (clone 1B6A3) (CAT#: VS7-0425-WR225)
This product is a mouse IgG1 antibody that binds to human KLF1. The antibody 1B6A3 recognizes specific amino acid domain of human KLF1 (AA: 208-362). It can be used for IHC and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/1770_F2_2_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/1770_F2_2_selected.jpg
Figure 2 IF staining of human cell line K-562
(Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/1854_C3_2_red_green.jpg
(Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/1854_C3_2_red_green.jpg
Figure 3 Bone marrow
(Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/124163_B_6_4.jpg
(Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51850/124163_B_6_4.jpg
Figure 4 RNA cell line category: Group enriched (HEL, K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105610-KLF1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105610-KLF1
Specifications
- Immunogen
- Recombinant human KLF1 (AA: 208-362) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KLF1
- Species Reactivity
- Human
- Clone
- 1B6A3
- Molecule Class
- Hematopoietic-Specific Transcription Factor
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Anemia, congenital dyserythropoietic, 4A (CDAN4A); Anemia, congenital dyserythropoietic, 4B (CDAN4B)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KLF1; Kruppel-like factor 1 (erythroid); EKLF; INLU; CDAN4; HBFQTL6; Krueppel-like factor 1; erythroid Kruppel-like factor; erythroid krueppel-like transcription factor; erythroid-specific transcription factor EKLF; monoclonal antibody A3D8
- Gene ID
- 10661
- UniProt ID
- Q13351
- Long Name
- Kruppel Like Factor 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_006554.1
- Function
- Activator; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-382 | Recombinant Human Anti-Human KLF1 Antibody, clone T320 | IP, IF, ELISA | IgG |
| MOB-1380MZ | Recombinant Mouse Anti-Human KLF1 Antibody (clone 5G12) | WB | Mouse antibody |
| VS3-XY978 | Mouse Anti-KLF1 Recombinant Antibody (clone 3A10) | ELISA, WB, IHC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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