AbPlus™ Anti-KLF4 Magnetic Beads (VS-0724-YC96) (CAT#: VS-0724-YC96)
The AbPlus Anti-KLF4 Magnetic Beads (VS-0724-YC96) is an innovative affinity resin which is bound with anti-KLF4 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of KLF4 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg

Figure 2 High expression in esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg

Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg

Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg

Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg

Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-KLF4 specific antibody
- Target
- KLF4
- Immunogen
- A synthetic peptide corresponding to the N-terminus of the human KLF4.
- Target Species
- Human, Mouse
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
- Alternative Names
- Kruppel Like Factor 4; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; GKLF; EZF; Endothelial Kruppel-Like Zinc Finger Protein; Krueppel-Like Factor 4;
- Gene ID
- 9314
- UniProt ID
- O43474
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3037z | Mouse Anti-KLF4 Recombinant Antibody (clone 36H2) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
MRO-1997-CN | Rabbit Anti-KLF4 Polyclonal Antibody (MRO-1997-CN) | WB, IHC, FC | Rabbit IgG |
ZG-0245C | Mouse Anti-KLF4 Recombinant Antibody (ZG-0245C) | WB | Mouse IgG |
VS3-CJ33 | Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ33) | WB, ELISA | Mouse IgG |
VS3-XY980 | Mouse Anti-KLF4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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