Mouse Anti-KLF4 Recombinant Antibody (VS-0924-YC36) - Cancer Stem Cell Marker
CAT#: VS-0924-YC36
This antibody panel includes the anti-KLF4 recombinant antibody (VS-0924-YC61) and the anti-KLF4 recombinant antibody (VS-0924-YC62). These antibodies specifically target the human KLF4 protein, which is a marker of cancer stem cells (CSCs). They are suitable for the detection of KLF4 in CSC tissues or cells using various techniques such as Immunohistochemistry (1/1000-1/2500), Immunofluorescence (2-10 µg/mL), and Western Blot (1 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
❮
❯
| Sub Cat | Type | |
| VS-0924-YC61 | Mouse IgG2b | |
| VS-0924-YC62 | Mouse IgG1 |
Specifications
- Host Species
- Mouse
- Specificity
- Human KLF4
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/1000-1/2500
Western Blot (WB): 1 µg/mL
Immunofluorescence (IF): 2-10 µg/mL
Target
- Alternative Names
- Kruppel Like Factor 4; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; GKLF; EZF; Endothelial Kruppel-Like Zinc Finger Protein; Krueppel-Like Factor 4
- Gene ID
- 9314
- UniProt ID
- O43474
- Long Name
- Kruppel like factor 4
- Cellular Localization
- Nucleus
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KLF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3037z | Mouse Anti-KLF4 Recombinant Antibody (clone 36H2) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1026CT | Recombinant Mouse anti-Human KLF4 Monoclonal antibody (2F6) | ELISA, ICC, IF, IHC-P, WB | |
| MRO-0901-CN | Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (JF98-08) | WB, IF, IHC | Rabbit IgG |
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| VS13-YC626 | Rabbit Anti-KLF4 Recombinant Antibody (VS13-YC626) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1956 | Hi-Affi™ Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (DS1956AB) | ELISA, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC96 | AbPlus™ Anti-KLF4 Magnetic Beads (VS-0724-YC96) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC63 | Rabbit Anti-KLF4 Antibody (VS-0924-YC63) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3795 | Anti-KLF4 Immunohistochemistry Kit | IHC |
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